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From 2013.igem.org

Restriction digest of LV3B,3C,3D,5A,5B and LV8A

A miniprep of these constructs was done by the use of the QuiaPrep Kit. Measurements via nanodrop displayed the following concentrations:

Construct	Concentration [ng/µl]	A260/280
LV3B	240,5	4,809/2,51
LV3C	170,3	3,41/1,81
LV3D	197,8	3,96/2,07
LV5A	212,6	4,25/1,256
LV5B	202,7	4,05/2,13
LV8A	40,1	0,802/0,44

These DNA fragments were digested with PstI. The Constructs 3B,3D 5B and 8A showed the desired bands. Hence, we transformed them in NEB-Turbo cells, by co-transformation with a PPTase. Some of the colonies (especially the colonies of LV8A) turned blue on the plates. Liquid cultures were prepared from all colonies we picked and induced with IPTG.