Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 11th September.html
From 2013.igem.org
Trojan Horse
ASDF11th September
![](https://static.igem.org/mediawiki/2013/d/d2/ParisB2013TH_11_09.png)
Results experiment Silencing
![](https://static.igem.org/mediawiki/2013/7/7b/ParisB2013TH_11_09_2.png)
![](https://static.igem.org/mediawiki/2013/0/09/ParisB2013TH_11_09_3.png)
![](https://static.igem.org/mediawiki/2013/f/f4/ParisB2013TH_11_09_4.png)
AT a concentration of 500mg/ul, we get 99,1% of killing (on 12 replicates) with a standard déviation of 1,19
![](https://static.igem.org/mediawiki/2013/7/78/ParisB2013TH_11_09_5.png)
-
When you try to understand why are the cells surviving, it is mainly due to the non silencing of the cells
-
This problem can be due to the necessity of the sRNA to match to the plasmid sequence perfectly (and therefore problemsof mutations, especially on phage).
![](https://static.igem.org/mediawiki/2013/4/42/ParisB2013TH_11_09_6.png)