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04/09/13
From 2013.igem.org
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*The double digest (pSB1C3) from yesterday was run on a 1% gel. | *The double digest (pSB1C3) from yesterday was run on a 1% gel. | ||
*This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector. | *This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector. | ||
| - | [[File:]] | + | [[File:igem_psb1c3_doubledigest_040913.jpg]] |
==Gel purification of the pSB1C3 backbone and RFP biobrick== | ==Gel purification of the pSB1C3 backbone and RFP biobrick== | ||
Revision as of 08:37, 5 September 2013
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Transformation results
Gel Electrophoresis
- The double digest (pSB1C3) from yesterday was run on a 1% gel.
- This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector.
Gel purification of the pSB1C3 backbone and RFP biobrick
- The Zymoclean Gel DNA recovery Kit was used to perform the purification and its protocol was followed.
- However the elution step was changed to 12ul of elution buffer, and this step was repeated.
