10/09/13

From 2013.igem.org

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==Dissolution of polystyrene==
==Dissolution of polystyrene==
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*Today a 20ml solution of 50% Limonene would be made to determine if the dissolution from [[09/09/13]] would occur faster.
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*Today a 20ml solution of 50% Limonene would be made to determine if the dissolution of polystyrene from [[09/09/13]] would occur faster.
==Gel electrophoresis==
==Gel electrophoresis==
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* The samples from the double digest on [[09/09/13]] were run to confirm if the digestion worked and also the cells with the ligated pSB1C3/TOD genes.
+
* The samples from the double digest on [[09/09/13]] were run to confirm if the digestion worked.
 +
* From the results, we would also be able to distinguish between cells that have been successfully transformed with the ligated pSB1C3/TOD genes from those with the recircularised pSB1C3.

Revision as of 10:02, 10 September 2013

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Dissolution of polystyrene

  • Today a 20ml solution of 50% Limonene would be made to determine if the dissolution of polystyrene from 09/09/13 would occur faster.

Gel electrophoresis

  • The samples from the double digest on 09/09/13 were run to confirm if the digestion worked.
  • From the results, we would also be able to distinguish between cells that have been successfully transformed with the ligated pSB1C3/TOD genes from those with the recircularised pSB1C3.