11 July 2013

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    Today we performed TOPO TA cloning of our PCR products of DNA aptamers from SELEX cycle 8. For this procedure we used pGEMTII Vector. The following steps were done:
  1. Ligation reaction by inserting our DNAs into pGEM vector
  2. Transformation of pGEM vector into JM109 High Efficiency Competent Cells
  3. Plating cells on LB/Ampicillin/IPTG/X-Gal plates

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