12/08/13

From 2013.igem.org

(Difference between revisions)
(Running an agarose gel for sonication analysis)
(Running an agarose gel for sonication analysis)
Line 7: Line 7:
==Running an agarose gel for sonication analysis==
==Running an agarose gel for sonication analysis==
-
*To tubes 1-6, add 14ul of sterile water and 5ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
+
*To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
-
*Use 5ul of 1kb marker on both ends
+
*Use 5 ul of 1kb marker on both ends
*Add buffer so that wells in the tray and the tray itself are covered
*Add buffer so that wells in the tray and the tray itself are covered
-
*Run at 80V for an hour and a half (gel is 1.2%)
+
*Run at 80 V for an hour and a half (gel is 1.2%)

Revision as of 14:12, 13 August 2013

Sonicating herring sperm DNA

  • Pipetting out sonicated samples every 30mins
    • Pipette 4ul of DNA into Son 1-6 every 30mins
      • Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
      • Vortex each dilution thoroughly and centrifuge briefly.
  • Keep tubes on ice

Running an agarose gel for sonication analysis

  • To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
  • Use 5 ul of 1kb marker on both ends
  • Add buffer so that wells in the tray and the tray itself are covered
  • Run at 80 V for an hour and a half (gel is 1.2%)