20/08/13

From 2013.igem.org

(Difference between revisions)
(Created page with "==Making nutrient agar plates and broth== *Making up broth and agar solution to grow ''P. putida'' in. *For agar plates: **9.2g of nutrient agar medium **400ml of distilled water...")
(Plating out bacteria)
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*Freeze dried ''P. putida'' was rehydrated using 1ml of nutrient broth
*Freeze dried ''P. putida'' was rehydrated using 1ml of nutrient broth
*The culture was aseptically transferred back into the broth containing tube
*The culture was aseptically transferred back into the broth containing tube
-
*Tube was mixed
+
*Mix
*From that tube, colonies were streaked on 6 plates and left to incubate over night at 30C
*From that tube, colonies were streaked on 6 plates and left to incubate over night at 30C

Revision as of 12:41, 21 August 2013

Making nutrient agar plates and broth

  • Making up broth and agar solution to grow P. putida in.
  • For agar plates:
    • 9.2g of nutrient agar medium
    • 400ml of distilled water
  • For broth:
    • 3.2g of nutrient broth
    • 400ml of distilled water
  • Both were autoclaved for 45mins
  • Agar was poured onto 16 plates

Plating out bacteria

  • Freeze dried P. putida was rehydrated using 1ml of nutrient broth
  • The culture was aseptically transferred back into the broth containing tube
  • Mix
  • From that tube, colonies were streaked on 6 plates and left to incubate over night at 30C

Growing bacteria in broth

  • 100ml of autoclaved broth was added to 2 flasks each
  • The broth tube, containing the bacteria, was divided between flasks
  • Flask were left to incubate at 30C on a shaker overnight