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Revision as of 10:51, 2 September 2013 (view source) JoshMiddleton (Talk | contribs) ← Older edit		Revision as of 10:51, 2 September 2013 (view source) JoshMiddleton (Talk | contribs) (Undo revision 98598 by JoshMiddleton (talk)) Newer edit →
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	| RBS || 49.1		| RBS || 49.1
	|-		|-
-
-
-	==Digestion==
-
-
-	{| class="wikitable"
-	|-
-	! Part !! OmpF (Part A) !! B0034 (Part B) !! AMP Plasmid !! RFP (Control)
-	|-
-	| DNA || 12.4 ul || 9.4 ul || 10.0 ul || 5.0 ul
-	|-
-	| Non-nuclease Water || 3.6 ul || 6.6 ul || 5.5 ul || 11.0 ul
-	|-
-	| NEB Buffer || 2.5 ul || 2.5 ul || 2.5 ul || 2.5 ul
-	|-
-	| BSA || 0.5 ul || 0.5 ul || 0.5 ul || 0.5 ul
-	|-
-	| EcoR1 || 0.5 ul || - || 0.5 ul || 0.5 ul
-	|-
-	| Spe1 || 0.5 || - || - || -
-	|-
-	| Xbal1 || - || 0.5 ul || - || -
-	|-
-	| Pst1 || - || 0.5 ul || 0.5 ul || 0.5 ul
-	|-
-	| Dpn1 || - || - || 0.5 ul || -
-	|}

---

Revision as of 10:51, 2 September 2013

Minipreps

We miniprepped:

• 1 + 4

• K592011 B

• K864404

• Cph8 #3

• 2 + 4

• K592022 B

• ENvZ no antibiotic

• ENvZ

• Cph8 #5

The liquid cultures of K592011 A and K592022 A did not grow.

NanoDrop from miniprep

Part	Concentration (ng/ul)
K592011 B	164.2
B0015	37.7
OmpR promoter	50.7
RBS	49.1