Exeter/29 August 2013

From 2013.igem.org

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==NanoDrop==
==NanoDrop==
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We collected NanoDrop data of previously miniprepped, defrosted DNA.
 
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
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*OmpF A - 20.1 ng/ul
+
*K592011 - 164.2 ng/ul
-
*OmpF B - 9.3ng/ul
+
*B0015 - 37.7 ng/ul
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*B0034 - 26.7ng/ul
+
*OmpR prom - 50.7 ng/ul
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*RFP - 198ng/ul
+
*RBS - 49.1 ng/ul
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The concentration for OmpF B was too low, so we continued on to the digestion with only OmpF A.
 
-
The concentration for RFP was too high, so we diluted it.
 
*RFP (dilution) - 50.4ng/ul
*RFP (dilution) - 50.4ng/ul

Revision as of 11:10, 2 September 2013

Minipreps

We miniprepped:

  • 1 + 4
  • K592011 B
  • K864404
  • Cph8 #3
  • 2 + 4
  • K592022 B
  • ENvZ no antibiotic
  • ENvZ
  • Cph8 #5


The liquid cultures of K592011 A and K592022 A did not grow.


NanoDrop

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

  • K592011 - 164.2 ng/ul
  • B0015 - 37.7 ng/ul
  • OmpR prom - 50.7 ng/ul
  • RBS - 49.1 ng/ul


  • RFP (dilution) - 50.4ng/ul

Digestion

Part OmpF (Part A) B0034 (Part B) AMP Plasmid RFP (Control)
DNA 12.4 ul 9.4 ul 10.0 ul 5.0 ul
Non-nuclease Water 3.6 ul 6.6 ul 5.5 ul 11.0 ul
NEB Buffer 2.5 ul 2.5 ul 2.5 ul 2.5 ul
BSA 0.5 ul 0.5 ul 0.5 ul 0.5 ul
EcoR1 0.5 ul - 0.5 ul 0.5 ul
Spe1 0.5 - - -
Xbal1 - 0.5 ul - -
Pst1 - 0.5 ul 0.5 ul 0.5 ul
Dpn1 - - 0.5 ul -