Exeter/29 August 2013

From 2013.igem.org

(Difference between revisions)
Line 34: Line 34:
*RBS - 49.1 ng/ul
*RBS - 49.1 ng/ul
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+
Two different RFP controls were nanodropped
 +
*RFP control - 75.9 ng/ul
 +
*RFP control - 61.7 ng/ul
==Digestion==
==Digestion==

Revision as of 11:21, 2 September 2013

Minipreps

We miniprepped:

  • 1 + 4
  • K592011 B
  • K864404
  • Cph8 #3
  • 2 + 4
  • K592022 B
  • ENvZ no antibiotic
  • ENvZ
  • Cph8 #5


The liquid cultures of K592011 A and K592022 A did not grow.


NanoDrop

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

  • K592011 B - 164.2 ng/ul
  • B0015 - 37.7 ng/ul
  • OmpR prom - 50.7 ng/ul
  • RBS - 49.1 ng/ul

Two different RFP controls were nanodropped

  • RFP control - 75.9 ng/ul
  • RFP control - 61.7 ng/ul

Digestion

Part K592011 (Part A) B0015 (Part B) AMP Plasmid RFP (Control)
DNA 1.52 ul 6.63 ul 10.0 ul 4.05 ul
Non-nuclease Water 14.48 ul 9.37 ul 5.5 ul 11.95 ul
NEB Buffer 2.5 ul 2.5 ul 2.5 ul 2.5 ul
BSA 0.5 ul 0.5 ul 0.5 ul 0.5 ul
EcoR1 0.5 ul - 0.5 ul 0.5 ul
Spe1 0.5 - - -
Xbal1 - 0.5 ul - -
Pst1 - 0.5 ul 0.5 ul 0.5 ul
Dpn1 - - 0.5 ul -


Part OmpR (Part A) RBS (Part B) AMP Plasmid RFP (Control)
DNA 4.93 ul 5.09 ul 10.0 ul 3.30 ul
Non-nuclease Water 11.07 ul 10.91 ul 5.5 ul 12.70 ul
NEB Buffer 2.5 ul 2.5 ul 2.5 ul 2.5 ul
BSA 0.5 ul 0.5 ul 0.5 ul 0.5 ul
EcoR1 0.5 ul - 0.5 ul 0.5 ul
Spe1 0.5 - - -
Xbal1 - 0.5 ul - -
Pst1 - 0.5 ul 0.5 ul 0.5 ul
Dpn1 - - 0.5 ul -