User contributions
From 2013.igem.org
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- 20:08, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/15 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Analysis of the sequencing resutls of the three promoters (sensing-module)''' <BR> The sequencing results were rather promising exce...")
- 20:04, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/14 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Making Buffers for the functional Assays''' <BR> In order to test the sensing module I wanted to let the bacteria grow in media with...")
- 19:59, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/13 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Innoculation of Bacteria with our constructs''' <BR> In order to characterize both the sensing and the effector plasmid I innoculate...")
- 19:57, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/12 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Test PCR for the constitutive promoter and the GelE insert''' <BR> I did a PCR of the MiniPrep and it showed that the plasmid did in...")
- 19:55, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/11 September 2013
- 19:53, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 19:52, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/10 September 2013
- 19:50, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/11 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of hya-construct (sensing), MMP2-construct (effector) and MMP9-construct (effector)''' <BR> .After the Minipreps of the innocuat...")
- 19:47, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/10 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''DpnI digest of the iGEM promoter and the GelE Backbone''' <BR> I digested the newly amplified and purified PCR products. The concent...")
- 19:39, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/9 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gibson Assembly of the hya-construct (sensing), the MMP2+GFP construct and the MMP9+GFP construct (effector)''' <BR> I did a Gibson ...")
- 19:32, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/8 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of MMP9 insert and GelE insert''' <BR> I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave...")
- 19:15, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/7 September 2013
- 19:02, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/7 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Purification of PCR products of the three sensing constructs''' <BR> I purified the PCR products from the previous Day and the conce...")
- 18:54, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/6 September 2013
- 18:54, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/6 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR> I did a 50ul PCR reaction of the Backbone and...")
- 18:51, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/5 September 2013
- 18:48, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/5 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM''' <BR> We used the same program as the day before in ...")
- 18:45, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 18:43, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 18:42, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 18:42, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/4 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Results of the gradient PCR of the Sensing constructs from the previous day''' <BR> The Gradient PCR of the Backbones for constructs...")
- 18:40, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/3 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gradient PCR of sensning constructs''' <BR> We used the new primers to run a PCR on the backbones and insert of the sensning constru...")
- 18:39, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/1 September 2013
- 18:36, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/1 September 2013
- 18:35, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/1 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Suspending Primers''' <BR> Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one...")
- 18:25, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/30 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Isolation of genomic MG1655 DNA''' <BR> We followed the manufactures instruction from the DNa isolation kit we recieved in order to ...")
- 18:24, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/29 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Results of GFP expression''' <BR> The inoculated bacteria did not grow at all in the arabinose containing medium. We thus innoculate...")
- 18:22, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/28 August 2013
- 18:21, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/28 August 2013
- 18:19, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/28 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Checking GFP expression induced by arabinose''' <BR> We wanted to induce simple GFP expression in bacteria transformed with the AraC...")
- 18:17, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/27 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Primer design''' <BR> We desinged new primers for the three sensing construct as well as for the three effector constructs. This tim...")
- 18:14, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/26 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the BBa_J23119 part from iGEM''' <BR> In order to have a positive control for our sensing constructs we wanted to use a const...")
- 18:11, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/25 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of Cad Backbone (1.2-B)''' <BR> We tried to amplify the Backbone for the Cad-GFP construct. But it did not work. Since the restr...")
- 18:08, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/23 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the effector Backbones''' <BR> Again we tried to amplifiy the backbones of the constructs containing the AraC promoter and th...")
- 18:04, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/22 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gradient PCR of Sensing Backbones''' <BR> We did another gradient PCR for the backbones but for the constructs three sensing constru...")
- 18:02, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/21 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Transformation of Bacteria''' <BR> We did another transformation: <BR> 1.) Two plates with the AraC promoter containing Plasmid, dir...")
- 18:00, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/20 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gradient PCR of Backbones''' <BR> To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR...")
- 17:59, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/19 August 2013
- 17:58, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/19 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gel electrophoresis of the backbones with and without GFP''' <BR> After running the gel for 1.5h, there were two visible bands for t...")
- 17:56, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/18 August 2013
- 17:54, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/18 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of MMP2 and MMP9 inserts and Backbones''' <BR> We did the same PCR as the previous day. It again did not work neither for the in...")
- 17:43, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/17 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gel Electrophoresis''' <BR> The PCR products from the day befor were run on a 0.8% agar gel.(see Results) The MMP inserts were not ...")
- 17:42, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/16 August 2013
- 17:41, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/16 August 2013
- 17:41, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/16 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Making a glycerol stock''' <BR> From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 50...")
- 17:34, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/15 August 2013
- 17:33, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/15 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Primer Resuspension''' <BR> We recieved our primers and resuspended them. Then we dilluted them in order to have a final concentrati...")
- 17:31, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/14 August 2013
- 17:30, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/14 August 2013
- 17:27, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/14 August 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Amplifying Plasmids containing gelatinases''' <BR> We transformed two cultures each with a plasmid containing a gene that encoded ei...")
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