Team:EPF Lausanne/Calendar/20 August 2013


Taxi.Coli: Smart Drug Delivery iGEM EPFL


Cell Surface Display

Gibson Assembly
- We did the streptavidin alive gibson assembly reaction (NEB protocol).
- Verification of the gibson reaction with a 0.8% gel electrophoresis.
- Gibson Assembly probably didn't work.


Gradient PCR of Backbones
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the right size.


Making Nanoparticles, 2nd try
- Biotinylation of the new nanoparticles.
- Dialysis of biotinylated nanoparticles.