Team:Arizona State/Adjuvants

From 2013.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 12: Line 12:
</script>
</script>
<body>
<body>
 +
<p>
 +
Adjuvants are an important component of vaccine design that triggers a stronger immune response in patients. The adjuvants designed for the BactoVax system are detailed below:
 +
</p>
 +
 +
<h3>CpG Motifs</h3>
 +
<p>
 +
Synthetic CpG Oligodeoxynucleotides are short single-stranded synthetic DNA molecules that contain a cytosine triphosphate deoxynucleotide ("C)" followed by a guanine triphosphate deoxynucleotide ("G"). Because they are unmethylated, they act as immunostimulants. Because bacterial chassis contain a large number of unmethylated CG motifs in their genome, they provide a strong natural immunostimulant in parallel with antigen delivery.
 +
</p>
 +
<h3>GM-CSF</h3>
 +
<p>
 +
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. It is a cytokine that acts as a white blood cell growth factor and stimulates growth of granulocytes. It also induces monocytes to activate into macrophages, making it a potent activator of the immune response. GM-CSF causes rapid multiplication of macrophage numbers near infection sites and has been used to stimulate white blood cell development post-chemotherapy. The ASU iGEM team is engineering GM-CSF to be expressed and secreted from the bacterial vaccine chassis to:
 +
<ol>
 +
<li>Recruit Immune Cells, including Dendritic Cells, to engulf the vaccine and present the antigens via the MHC Class I pathway. This creates an antigen-presenting cell-targeted vaccine system.</li>
 +
<li>Stimulate production of more immune cells to mount a stronger anti-tumor response.</li>
 +
<li>Act as a potential factor for translational applications of the vaccine system in conjunction with chemotherapy to replenish white blood cell levels.</li>
 +
</ol>
 +
</p>
-
<h3>GMCSF recruits Immune Cells to Site of Infection</h3>
 
</body>
</body>
</html>
</html>

Latest revision as of 02:20, 28 September 2013

Adjuvants are an important component of vaccine design that triggers a stronger immune response in patients. The adjuvants designed for the BactoVax system are detailed below:

CpG Motifs

Synthetic CpG Oligodeoxynucleotides are short single-stranded synthetic DNA molecules that contain a cytosine triphosphate deoxynucleotide ("C)" followed by a guanine triphosphate deoxynucleotide ("G"). Because they are unmethylated, they act as immunostimulants. Because bacterial chassis contain a large number of unmethylated CG motifs in their genome, they provide a strong natural immunostimulant in parallel with antigen delivery.

GM-CSF

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. It is a cytokine that acts as a white blood cell growth factor and stimulates growth of granulocytes. It also induces monocytes to activate into macrophages, making it a potent activator of the immune response. GM-CSF causes rapid multiplication of macrophage numbers near infection sites and has been used to stimulate white blood cell development post-chemotherapy. The ASU iGEM team is engineering GM-CSF to be expressed and secreted from the bacterial vaccine chassis to:

  1. Recruit Immune Cells, including Dendritic Cells, to engulf the vaccine and present the antigens via the MHC Class I pathway. This creates an antigen-presenting cell-targeted vaccine system.
  2. Stimulate production of more immune cells to mount a stronger anti-tumor response.
  3. Act as a potential factor for translational applications of the vaccine system in conjunction with chemotherapy to replenish white blood cell levels.