Team:BYU Provo/Large Phage

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:The goal of the Large Phage Project is to make a mutant phage that has a capsid much larger than the normal ~200nm T4 capsid. Our intent is to create a protocol where large phage with stable phenotypes will form making it possible to "pick a size" when using phage as a delivery system. The impact of these large phage could mean the difference in making a drug treatment effective and efficient.
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:Through the use of our mutagen 5'bromodeoxyuridine, a good mutagenesis protocol and the use of a sensitive cesium chloride gradient. We were able to mutagenize and isolate giant mutant t4 phage.
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Latest revision as of 19:26, 6 September 2013

LargePhagePic-1.JPG


Overview
March-April
May-June
July-August
September-October

Overview


    • still needs work
The goal of the Large Phage Project is to make a mutant phage that has a capsid much larger than the normal ~200nm T4 capsid. Our intent is to create a protocol where large phage with stable phenotypes will form making it possible to "pick a size" when using phage as a delivery system. The impact of these large phage could mean the difference in making a drug treatment effective and efficient.
Through the use of our mutagen 5'bromodeoxyuridine, a good mutagenesis protocol and the use of a sensitive cesium chloride gradient. We were able to mutagenize and isolate giant mutant t4 phage.

EMpic-1.jpg




Contents

September

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