Team:BYU Provo/Notebook/Phage Purification/Fallexp

From 2013.igem.org

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This marks the start of our small phage group! Although we are still on the background-research phase and not doing any wet-lab experiments, we are sure our work will be worthwhile. As Dr. Grose, our wonderful instructor, says "a month in the lab will say you a day in the library." After two weeks of hardcore literature search, we now have a clear idea of where our project is headed. And we are super excited to get started!
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We have recently had many problems getting the T7 bacteriophage to band.  We focussed on trying to discover what the problem was and how we could fix it. We think it could have been due to a bad batch of phage suspension buffer, and our results confirmed it!
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/PR|Presentation]] </font>
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| <font size="5" font face="Calibri"> '''September 1 - September 14''' </font>
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| <font size="5" font face="Calibri"> '''September 15 - September 30''' </font>
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<font size="3" font face="Calibri"> After several attempts, T7  has  finally banded! We discovered that the band may disappear because the T7 band is faint, even at very high titer. Mutated phage is low titer, and if the gradient is made more specific, the band would be impossible to see. With this knowledge, we can continue on with purification. These two weeks are the last weeks of experiments, and the latter week was devoted to working on the wiki.  </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period2/Dailylog|Daily log]]
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<font size="5" font face="Calibri"> '''October 1- October 6''' </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period2/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period2/PR| Progress Report]] </font>
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Competed at regional iGEM!
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<font size="5" font face="Calibri"> '''October 7 - 13''' </font>
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Helped out groups that needed it. See other groups pages for details.
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| <font size="5" font face="Calibri"> '''September 15 - September 30''' </font>
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<font size="5" font face="Calibri"> '''October 14 - 20''' </font>
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Helped out groups that needed it. Took TEM of Small Phage T7.
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Explist|Experiment Listing]]
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<font size="5" font face="Calibri"> '''October 21 - 27''' </font>
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Ran PCR for T4
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period3/PR| Progress Report]] </font>
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Latest revision as of 20:48, 21 October 2013


Phage Purification September - October Notebook



Phage Purification
March-April
May-June
July-August
September-October

<< Previous

September 1 - September 14


We have recently had many problems getting the T7 bacteriophage to band. We focussed on trying to discover what the problem was and how we could fix it. We think it could have been due to a bad batch of phage suspension buffer, and our results confirmed it!



Daily log

Experiment Listing


Darrenworking2.jpg


September 15 - September 30


After several attempts, T7 has finally banded! We discovered that the band may disappear because the T7 band is faint, even at very high titer. Mutated phage is low titer, and if the gradient is made more specific, the band would be impossible to see. With this knowledge, we can continue on with purification. These two weeks are the last weeks of experiments, and the latter week was devoted to working on the wiki.



October 1- October 6


Competed at regional iGEM!




October 7 - 13


Helped out groups that needed it. See other groups pages for details.



October 14 - 20


Helped out groups that needed it. Took TEM of Small Phage T7.


Daily log

Experiment Listing



October 21 - 27


Ran PCR for T4


Daily log

Experiment Listing


<< Previous


Winter3.JPG

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