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Team:BYU Provo/Notebook/Phage Purification/Fallexp
From 2013.igem.org
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| - | + | We have recently had many problems getting the T7 bacteriophage to band. We focussed on trying to discover what the problem was and how we could fix it. We think it could have been due to a bad batch of phage suspension buffer, and our results confirmed it! | |
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Explist|Experiment Listing]] | [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Explist|Experiment Listing]] | ||
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| - | | style="width: 20%; background-color: transparent;"| [[File: | + | | style="width: 20%; background-color: transparent;"| [[File:Darrenworking2.jpg|220px|center]] |
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| - | | <font size="5" font face="Calibri"> '''September | + | | <font size="5" font face="Calibri"> '''September 15 - September 30''' </font> |
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> After several attempts, T7 has finally banded! We discovered that the band may disappear because the T7 band is faint, even at very high titer. Mutated phage is low titer, and if the gradient is made more specific, the band would be impossible to see. With this knowledge, we can continue on with purification. These two weeks are the last weeks of experiments, and the latter week was devoted to working on the wiki. </font> |
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| - | + | <font size="5" font face="Calibri"> '''October 1- October 6''' </font> | |
| - | + | <br> | |
| - | + | <font size="3" font face="Calibri"> | |
| + | Competed at regional iGEM! | ||
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| + | </font> | ||
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| + | <br> | ||
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| + | <font size="5" font face="Calibri"> '''October 7 - 13''' </font> | ||
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| - | + | Helped out groups that needed it. See other groups pages for details. | |
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| - | + | </font> | |
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| + | <font size="5" font face="Calibri"> '''October 14 - 20''' </font> | ||
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> |
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| + | Helped out groups that needed it. Took TEM of Small Phage T7. | ||
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| + | </font> | ||
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| + | <font color="#333399" size="4" font face="Calibri"> | ||
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| + | [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Dailylog|Daily log]] | ||
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| + | [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Explist|Experiment Listing]] | ||
| + | |||
| + | </font> | ||
<br> | <br> | ||
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| + | <font size="5" font face="Calibri"> '''October 21 - 27''' </font> | ||
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| + | <br> | ||
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| + | <font size="3" font face="Calibri"> | ||
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| + | Ran PCR for T4 | ||
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| + | </font> | ||
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[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period3/Explist|Experiment Listing]] | [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period3/Explist|Experiment Listing]] | ||
| - | + | </font> | |
<br> | <br> | ||
Latest revision as of 20:48, 21 October 2013
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September 1 - September 14
We have recently had many problems getting the T7 bacteriophage to band. We focussed on trying to discover what the problem was and how we could fix it. We think it could have been due to a bad batch of phage suspension buffer, and our results confirmed it!
| 
|
| September 15 - September 30
After several attempts, T7 has finally banded! We discovered that the band may disappear because the T7 band is faint, even at very high titer. Mutated phage is low titer, and if the gradient is made more specific, the band would be impossible to see. With this knowledge, we can continue on with purification. These two weeks are the last weeks of experiments, and the latter week was devoted to working on the wiki.
October 1- October 6
Competed at regional iGEM!
October 7 - 13
Helped out groups that needed it. See other groups pages for details.
October 14 - 20
Helped out groups that needed it. Took TEM of Small Phage T7.
October 21 - 27
Ran PCR for T4
| 
| |
