Team:BYU Provo/Notebook/Phage Purification/Springexp/Period5/Dailylog

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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="4"> '''6/10/13''' </font>
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<font size="4"> '''6/24/13''' </font>
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- Prepared RNase A for future use.
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- Finished purification with a CsCl gradient and extracted phage.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10RNasePreparation|6.10 RNase A Preparation]]
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.24CsClGradient|6.24 CsCl Gradient]]
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- Prepared T4 and T7 cultures.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]
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<font size="4"> '''6/12/13''' </font>
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<font size="4"> '''6/26/13''' </font>
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- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]].
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
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<font size="4"> '''6/14/13''' </font>
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- Worked on updating the website.
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<font size="4"> '''6/17/13''' </font>
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- Set up CsCl gradient
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]]
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]]
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<font size="4"> '''6/19/13''' </font>
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- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]].
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]]
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- Ran spot tests on collected band from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]].
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Exp/6.19Spot Test|6.19 Spot Test]]
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<font size="4"> '''6/20/13''' </font>
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- Performed a titer on purified phage to test the concentration.
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- Finished [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] by centrifuging solution at 10,000 g 15 minutes at 4<sup>◦</sup> C, removing supernatant, and suspending the pellet in phage suspension buffer.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.26T7Titer|6.26 T7 Titer]]
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<font size="4"> '''6/21/13''' </font>
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<font size="4"> '''6/28/13''' </font>
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We centrifuged the pellet at 10,000 g for 15 min at 4<sup>◦</sup> C after suspending it to clean up the walls of the tube.
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- The large phage group switched to using E. coli B instead of W3110.  We will now be using E. coli B instead of W3110.  Streaked plates of BL21 and B for future use.  Left in the 37<sup>◦</sup> C over the weekend.
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Latest revision as of 00:04, 28 September 2013


Phage Purification May - June Notebook: June 24 - June 30 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



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6/24/13

- Finished purification with a CsCl gradient and extracted phage.

6.24 CsCl Gradient


6/26/13

- Performed a titer on purified phage to test the concentration.

6.26 T7 Titer


6/28/13

- The large phage group switched to using E. coli B instead of W3110. We will now be using E. coli B instead of W3110. Streaked plates of BL21 and B for future use. Left in the 37 C over the weekend.


<< Previous Next >>