Team:BYU Provo/Notebook/Phage Purification/Springexp/Period5/Dailylog

- The large phage group switched to using E. coli B instead of W3110. We will now be using E. coli B instead of W3110. Streaked plates of BL21 and B for future use. Left in the 37^◦ C over the weekend.

6/17/13

- Set up CsCl gradient 6.17 CsCl Gradient

- Prepared T4 and T7 cultures.

6.17 Phage Preparation

6/19/13

- Began first step of purification process using phage from 6.17 Phage Preparation.

6.19 PEG Purification

- Ran spot tests on collected band from 6.17 CsCl Gradient.

6.19 Spot Test

6/20/13

- Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4^◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.

6/21/13

We centrifuged the pellet at 10,000 g for 15 min at 4^◦ C after suspending it to clean up the walls of the tube.

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-<font size="4"> '''6/12/13''' </font>
+<font size="4"> '''6/26/13''' </font>
-- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]].
+- Performed a titer on purified phage to test the concentration.
 : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
@@ Line 58: / Line 58: @@
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-<font size="4"> '''6/14/13''' </font>
+<font size="4"> '''6/28/13''' </font>
-- Worked on updating the website.
+- The large phage group switched to using E. coli B instead of W3110.  We will now be using E. coli B instead of W3110.  Streaked plates of BL21 and B for future use.  Left in the 37<sup>◦</sup> C over the weekend.
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