Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.31 EM Pictures

(Difference between revisions)

Revision as of 21:01, 20 September 2013

Phage Purification July - August Notebook: Experiments

7.31 EM Pictures

I) Purpose

To see if we have mutated, pure phage from the CsCl gradient and mutagenisis

II) Expected Outcome

Pictures of pure, intact phage, potentially mutated phage.

III) Reagants Used

Prepared Phage

IV) Actual Procedure

Gave prepared phage to TEM operator. We had four sample types:

Phage from the 'petite' band in the CsCl gradient

Phage from the 'large phage' band in the CsCl gradient

V) Results

Pictures of Phage

Petite Phage

.

Large Phage

@@ Line 48: / Line 48: @@
 : Gave prepared phage to TEM operator. We had four sample types:
 :Phage from the 'petite' band in the CsCl gradient
-:Phage from the 'regular/wildtype' band in the CsCl gradient
+:Phage from the 'large phage' band in the CsCl gradient
-:Phage from the 'mutant' band in the CsCl gradient
-:Phage that had 2x mutagen
 '''V) Results'''
@@ Line 62: / Line 61: @@
 [[File:Phage31_6.jpg | thumb|none|alt=]]
 [[File:Phage31_7.jpg | thumb|none|alt=]]
-:Wild Type Phage
+:Large Phage
 [[File:Phage31_wt1.jpg | thumb|none|alt=]]
 [[File:Phage31_wt2.jpg | thumb|none|alt=]]
@@ Line 71: / Line 70: @@
 [[File:Phage31_wt7.jpg | thumb|none|alt=]]
 [[File:Phage31_wt8.jpg | thumb|none|alt=]]
-:Mutated Phage
-[[File:Phage31_m1.jpg | thumb|none|alt=]]
-[[File:Phage31_m2.jpg | thumb|none|alt=]]
-[[File:Phage31_m3.jpg | thumb|none|alt=]]
-[[File:Phage31_m4.jpg | thumb|none|alt=]]
-[[File:Phage31_m5.jpg | thumb|none|alt=]]
-[[File:Phage31_m6.jpg | thumb|none|alt=]]
-:Mutated Phage - 2x Mutagen
-[[File:Phage31_m21.jpg | thumb|none|alt=]]
-[[File:Phage31_m22.jpg | thumb|none|alt=]]
-[[File:Phage31_m23.jpg | thumb|none|alt=]]
-[[File:Phage31_m24.jpg | thumb|none|alt=]]
-[[File:Phage31_m25.jpg | thumb|none|alt=]]
-[[File:Phage31_m26.jpg | thumb|none|alt=]]
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