Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.31 EM Pictures

From 2013.igem.org

(Difference between revisions)

Latest revision as of 00:35, 28 September 2013

Phage Purification July - August Notebook: Experiments

Phage Purification

7.31 EM Pictures

I) Purpose

To see if we have mutated, pure phage from the CsCl gradient and mutagenisis

II) Expected Outcome

Pictures of pure, intact phage, potentially mutated phage.

III) Reagants Used

Prepared Phage

IV) Actual Procedure

Gave prepared phage to TEM operator. We had four sample types:

Phage from the 'petite' band in the CsCl gradient

Phage from the 'large phage' band in the CsCl gradient

V) Results

Pictures of Phage

Petite Phage

Large Phage

@@ Line 10: / Line 10: @@
 |- valign="top"
-| style="width: 18%; background-color: transparent;"|
+| style="width: 20%; background-color: transparent;"|
 <font color="#333399" size="3" font face="Calibri">
-: [[Team:BYU_Provo/Phage_Purification|Overview]]
+<font size = "4">
+: <u> '''Phage Purification''' </u> </font>
 : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
@@ Line 26: / Line 28: @@
 </font>
-| style="width: 82%; background-color: transparent;"|
+| style="width: 80%; background-color: transparent;"|
 <font face="Calibri" size="3">
@@ Line 48: / Line 50: @@
 : Gave prepared phage to TEM operator. We had four sample types:
 :Phage from the 'petite' band in the CsCl gradient
-:Phage from the 'regular/wildtype' band in the CsCl gradient
+:Phage from the 'large phage' band in the CsCl gradient
-:Phage from the 'mutant' band in the CsCl gradient
-:Phage that had 2x mutagen
 '''V) Results'''
@@ Line 56: / Line 57: @@
 :Petite Phage
 [[File:Phage31_1.jpg | thumb|none|alt=]]
-[[File:Phage31_2.jpg | thumb|none|alt=]].
+[[File:Phage31_2.jpg | thumb|none|alt=]]
 [[File:Phage31_3.jpg | thumb|none|alt=]]
 [[File:Phage31_4.jpg | thumb|none|alt=]]
@@ Line 62: / Line 63: @@
 [[File:Phage31_6.jpg | thumb|none|alt=]]
 [[File:Phage31_7.jpg | thumb|none|alt=]]
-:Wild Type Phage
+:Large Phage
 [[File:Phage31_wt1.jpg | thumb|none|alt=]]
 [[File:Phage31_wt2.jpg | thumb|none|alt=]]
@@ Line 71: / Line 72: @@
 [[File:Phage31_wt7.jpg | thumb|none|alt=]]
 [[File:Phage31_wt8.jpg | thumb|none|alt=]]
-:Mutated Phage
-[[File:Phage31_m1.jpg | thumb|none|alt=]]
-[[File:Phage31_m2.jpg | thumb|none|alt=]]
-[[File:Phage31_m3.jpg | thumb|none|alt=]]
-[[File:Phage31_m4.jpg | thumb|none|alt=]]
-[[File:Phage31_m5.jpg | thumb|none|alt=]]
-[[File:Phage31_m6.jpg | thumb|none|alt=]]
-:Mutated Phage - 2x Mutagen
-[[File:Phage31_m21.jpg | thumb|none|alt=]]
-[[File:Phage31_m22.jpg | thumb|none|alt=]]
-[[File:Phage31_m23.jpg | thumb|none|alt=]]
-[[File:Phage31_m24.jpg | thumb|none|alt=]]
-[[File:Phage31_m25.jpg | thumb|none|alt=]]
-[[File:Phage31_m26.jpg | thumb|none|alt=]]
 </font>