Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.3 EM Phage Preparation
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+ | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : | + | : 300 mesh copper grid |
+ | : 2% phosphotungstic acid | ||
+ | : filter paper | ||
+ | : petri dish | ||
+ | : T4 lysate | ||
+ | : parafilm | ||
'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
- | : | + | : Place parafilm in the petri dish and put a drop of T4 lysate and a drop of 2% phosphotungstic acid on the parafilm. |
+ | : Place the 300 grid mesh copper grid on the T4 lysate drop and leave for 20 minutes. | ||
+ | : Remove the grid and place on the 2% phosphotungstic acid drop and leave for 2 minutes. | ||
+ | : Remove the grid and dry with filter paper by lightly touching the tip to the grid. | ||
+ | : Place grid in the EM grid box. | ||
'''V) Results''' | '''V) Results''' |
Latest revision as of 00:31, 28 September 2013
Phage Purification July - August Notebook: Experiments
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7.3 EM Phage Preparation
I) Purpose
II) Expected Outcome
III) Reagants Used
V) Results
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