Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/5.10 Bacteria Cultures, 25 mL

From 2013.igem.org

(Difference between revisions)
(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: Experiments'''</font> <br>...")
 
Line 10: Line 10:
|- valign="top"
|- valign="top"
-
| style="width: 18%; background-color: transparent;"|
+
| style="width: 20%; background-color: transparent;"|
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Phage_Purification|Overview]]
+
<font size = "4">
 +
 
 +
: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
Line 26: Line 28:
</font>
</font>
-
| style="width: 82%; background-color: transparent;"|
+
| style="width: 80%; background-color: transparent;"|
<font face="Calibri" size="3">
<font face="Calibri" size="3">

Latest revision as of 00:25, 28 September 2013


Phage Purification May - June Notebook: Experiments



Phage Purification
March-April
May-June
July-August
September-October

5.10 Bacteria Cultures, 25 mL


I) Purpose

Grow bacteria for later use in purification of phage.

II) Expected Outcome

Viable liquid cultures of E. coli W3110

III) Reagants Used

W3110 freezer stock
LB


IV) Actual Procedure

Infect 25 mL of LB with 1 loop-full of bacterial freezer stock and leave in shaker at 37◦ overnight.

V) Results

Growth of liquid culture of bacteria to infect and amplify amounts of phage.


Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.10_Bacteria_Cultures,_25_mL"