Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.12PEGPurification
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'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
- | : Add | + | : Add 166.5 microL DNase and 22.5 microL RNase to T4 and T7 bacterial lysates. |
- | : Add . | + | : Add .0904 mL chloroform to complete lysis and let the preparations sit for 30 minutes at room temperature. |
- | : Dissolve 1. | + | : Dissolve 1.32 g of solid NaCl and let cool at 4<sup>◦</sup> C for 1 hour. |
: Centrifuge the suspensions at 6500 rpms (7000 g) for 10 minutes at 4<sup>◦</sup> C. Remove supernatant and put in clean flasks. | : Centrifuge the suspensions at 6500 rpms (7000 g) for 10 minutes at 4<sup>◦</sup> C. Remove supernatant and put in clean flasks. | ||
- | : Dissolve | + | : Dissolve 3.96 g PEG 8000 and let sit at 4<sup>◦</sup> C for 1 hour. |
: Centrifuge the suspensions at 8000 rpms (10000 g) for 15 minutes at 4<sup>◦</sup> C. Discard the supernatant. | : Centrifuge the suspensions at 8000 rpms (10000 g) for 15 minutes at 4<sup>◦</sup> C. Discard the supernatant. | ||
: Turn the centrifuge bottles over to let sit for 5 minutes to drain off any remaining supernatant. | : Turn the centrifuge bottles over to let sit for 5 minutes to drain off any remaining supernatant. |
Revision as of 20:33, 14 June 2013
Phage Purification May - June Notebook: Experiments
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5.26 PEG Purification
I) Purpose
II) Expected Outcome
III) Reagants Used
IV) Actual Procedure
V) Results
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