Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period9/Explist

From 2013.igem.org

(Difference between revisions)

Latest revision as of 00:35, 28 September 2013

Phage Purification July - August Notebook: July 16 - July 31 Experiment Listing

Phage Purification

7.17 CsCl Gradient

Trying out a new gradient for T4 purification.

Detailed procedure: 7.17 CsCl Gradient

7.26 T4 Titer

We ran a titer on our mutated phage from the CsCl gradient to make sure we had a high enough concentration of phage for an EM.

Detailed procedure: 7.26 Phage Titer

7.31 EM Pictures

These are the picture results from the EM.

7.31 EM Pictures

@@ Line 10: / Line 10: @@
 |- valign="top"
-| style="width: 18%; background-color: transparent;"|
+| style="width: 20%; background-color: transparent;"|
 <font color="#333399" size="3" font face="Calibri">
-: [[Team:BYU_Provo/Phage_Purification|Overview]]
+<font size = "4">
+: <u> '''Phage Purification''' </u> </font>
 : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
@@ Line 26: / Line 28: @@
 </font>
-| style="width: 82%; background-color: transparent;"|
+| style="width: 80%; background-color: transparent;"|
 <font face="Calibri" size="3">
-<font size="4"> '''7.3 EM Phage Preperation''' </font>
+<font size="4"> '''7.17 CsCl Gradient''' </font>
-: This is the procedure used to prepare samples of purified phage for electron microscopy imaging (EM).
+: Trying out a new gradient for T4 purification.
-: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.3 EM Phage Preparation |7.3 EM Phage Preparation]]
+: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.17 CsCl Gradient|7.17 CsCl Gradient]]
 <br>
-<font size="4"> '''7.5 Bacterial Preparation''' </font>
+<font size="4"> '''7.26 T4 Titer''' </font>
-: Streaking cultures of bacteria for use in phage propagation
+: We ran a titer on our mutated phage from the CsCl gradient to make sure we had a high enough concentration of phage for an EM.
-: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.5 Bacterial Preparation|7.5 Bacterial Preparation]]
+: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.26 Phage Titer |7.26 Phage Titer]]
 <br>
-<font size="4"> '''7.12 Bacterial Preparation''' </font>
+<font size="4"> '''7.31 EM Pictures''' </font>
-:Streaking cultures of bacteria for use in phage propagation
+: These are the picture results from the EM.
-:Detailed Procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.12 Bacterial Preparation|7.12 Bacterial Preparation]]
-<br>
-<font size="4"> '''7.17 CsCl Gradient''' </font>
-: Trying out a new gradient for T4 purification.
-: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.17 CsCl Gradient|7.17 CsCl Gradient]]
+: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.31 EM Pictures |7.31 EM Pictures]]
 <br>