Team:BYU Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry
From 2013.igem.org
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2) Repeat of the amplification and purification of insert (10.20) | 2) Repeat of the amplification and purification of insert (10.20) | ||
: Protocols of this experiment were similar to that of 1) Amplification and purification of insert (10.18), except for the following differences | : Protocols of this experiment were similar to that of 1) Amplification and purification of insert (10.18), except for the following differences | ||
- | * 10uL of phage and 40uL of ddH2O was used in the boiling process | + | :: * 10uL of phage and 40uL of ddH2O was used in the boiling process |
- | * extention time was changed to 3 miinutes | + | :: * extention time was changed to 3 miinutes |
- | * a regular gel was used to verify PCR product | + | :: * a regular gel was used to verify PCR product |
- | * 3uL of product and 3uL of loading dye was used per well in the gel. | + | :: * 3uL of product and 3uL of loading dye was used per well in the gel. |
'''V) Results''' | '''V) Results''' |
Revision as of 16:59, 21 October 2013
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10.18 Cloning T7 Capsid Protein into iGEM Registry
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Amplification and purification of insert (10.18)
2) Repeat of the amplification and purification of insert (10.20)
V) Results 1) Amplification and purification of insert
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