Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period2/Dailylog

From 2013.igem.org

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* Started approximately 20 mL of E coli B liquid culture overnight.LP
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* Started approximately 20 mL of E coli B liquid culture overnight.
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<font size="4"> '''9/5/13''' </font>
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<font size="4"> '''9/20/13''' </font>
LP
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* Started approximately 10mL of E coli B liquid culture overnight.
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* Performed "Plating to Check for Plaque Size" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
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<font size="4"> '''9/6/13''' </font>
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<font size="4"> '''9/23/13''' </font>
JL, LP
JL, LP
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* Purified phage propagation sample from 9.4 via centrifugation (5 minutes at 3000 rpm) and cholorform. Spot tests were then performed to estimate phage titer.
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* Performed "Plating to Check for Plaque Size 2" and "Checking for Plaque Viability from Step 5" for  [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
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* Took pictures of plates from [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]] using alpha imager.
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* Divided up assignments for updating notebook.
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<font size="4"> '''9/7/13''' </font>
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<font size="4"> '''9/24/13''' </font>
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JL
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LP
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* Started approximately 15 mL E coli B liquid culture overnight.
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* Started approximately 30 mL E coli B liquid culture overnight.
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* Worked on data analysis / picture processing for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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<font size="4"> '''9/8/13''' </font>
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<font size="4"> '''9/25/13''' </font>
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JL
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JL, LP
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* Continued data analysis / picture processing for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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* Performed "Checking for Plaque Viability 2" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
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* Performed preliminary titer in preparation for repeating the modeling experiment with T1 and T2. For specifics, please see  [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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* Started approximately 25mL of E coli B liquid culture overnight
* Started approximately 25mL of E coli B liquid culture overnight
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<font size="4"> '''9/9/13''' </font>
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<font size="4"> '''9/26/13''' </font>
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JL, LP
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LP
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* Discussed analysis of modeling result using ImageJ.
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* Started approximately 25mL of E. coli B overnight.
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* Repeated the modeling procedure in  [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]] for T1 and T2. Incubation started at 4:30pm.
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* Started a fresh round for phage propagation for the Phage Purification Team. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.
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* Divided up assignments for updating the wiki.
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<font size="4"> '''9/10/13''' </font>
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<font size="4"> '''9/27/13''' </font>
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JL, LP
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* Took the T1 and T2 modeling plates our the incubation at 4:30pm.
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* Started approximately 25mL of E coli B liquid culture overnight.
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* Measured plaque sizes using ImageJ.
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<font size="4"> '''9/11/13''' </font>
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JL, LP
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* Isolated the phage (from 9.9) by centrifuging down the bacteria, transferring the supernatant to a new 15mL centrifuge tube, and adding 900ul of chloroform to the supernatant.
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* Took pictures of T1 and T2 plates for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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<font size="4"> '''9/12/13''' </font>
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JL
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* Spot test showed plaques up to -7. This indicate that the phage suspension solution we gave the Phage Purification Team for CsCl was at least 10E9 pfu/mL.
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* Started approximately 30mL of E coli B liquid culture overnight.
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<font size="4"> '''9/13/13''' </font>
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JL, LP
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* The Phage Purification Team has good news for us. They were able to observe banding in their latest CsCl gradient for T7. We thus decided to perform a new round of mutagenesis this weekend and hopefully selection next week.
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* In preparation for mutagenesis tomorrow, we performed titers for 8.24 T7 stock.
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* Started approximately 5mL of E coli B liquid culture overnight.
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* Streaked out E coli B.
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<font size="4"> '''9/14/13''' </font>
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JL
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* Autoclaved ddH<sub>2</sub>O, LB, x2 top agar.
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* Used the autoclaved ddH<sub>2</sub>O to make adenine and uracil solution. Specifically,
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: - 254mg of uracil was dissolved in 10mL of ddH<sub>2</sub>O to produce a uracil solution with a concentration of approximately 2.5mg/mL
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: - 753mg of adenine hemisulfate dihydrate was dissolved in 10mL of ddH<sub>2</sub>O to produce a adenine solution with a concentration of approximately 5mg/mL
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* Started approximately 5mL of E coli B liquid culture overnight.
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<font size="4"> '''9/15/13''' </font>
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JL, LP
JL, LP
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* Performed the mutagenesis procedure in [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]]
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* Determined results for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
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* Started approximately 20mL of E coli B liquid culture overnight
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* Finished updating the wiki.
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* Started phage propagation
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* Started approximately 30mL of E. coli B overnight.
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Latest revision as of 03:16, 28 September 2013


Small Phage September - October Notebook: September 16 - September 30 Daily Log



Small Phage
March-April
May-June
July-August
September-October

9/16/13

JL, LP

  • Discussed plans for these future two weeks: established priority.
  • Help Large Phage Group with dilution series and performing a spot test for their small phage band.


9/17/13

LP

  • Started approximately 20mL of E coli B liquid culture overnight.


9/18/13

JL, LP

  • Discussed CsCl gradient set up with Phage Purification Team.


9/19/13

LP

  • Started approximately 20 mL of E coli B liquid culture overnight.


9/20/13

LP


9/23/13

JL, LP


9/24/13

LP

  • Started approximately 30 mL E coli B liquid culture overnight.


9/25/13

JL, LP

  • Started approximately 25mL of E coli B liquid culture overnight


9/26/13

LP

  • Started approximately 25mL of E. coli B overnight.


9/27/13

JL, LP

  • Finished updating the wiki.
  • Started approximately 30mL of E. coli B overnight.