Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period3/Dailylog

From 2013.igem.org

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<font size="4"> '''9/16/13''' </font>
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<font size="4"> '''10/1/13 - 10/3/13''' </font>
JL, LP
JL, LP
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* Discussed plans for these future two weeks: established priority.
+
* Worked on putting together Jamboree presentation and poster.  
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+
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* Help Large Phage Group with dilution series and performing a spot test for their small phage band.  
+
<br>
<br>
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<font size="4"> '''9/17/13''' </font>
+
<font size="4"> '''10/4/13 - 10/6/13''' </font>
-
LP
+
JL, LP
-
* Started approximately 20mL of E coli B liquid culture overnight.
+
* North America Regional Jamboree! For more click [[Team:BYU_Provo/Toronto|here]].
<br>
<br>
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<font size="4"> '''9/18/13''' </font>
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<font size="4"> '''10/7/13''' </font>
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JL, LP
+
JL
-
 
+
-
* Discussed CsCl gradient set up with Phage Purification Team.
+
-
* Performed dilution series and spot test for samples post CsCl from [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Started approximately 10 mL E coli B liquid culture overnight.
<br>
<br>
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<font size="4"> '''9/19/13''' </font>
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<font size="4"> '''10/8/13''' </font>
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LP
+
JL
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* Started approximately 20 mL of E coli B liquid culture overnight.
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* Started [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]] by propagating the mutants.
<br>
<br>
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<font size="4"> '''9/20/13''' </font>
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<font size="4"> '''10/9/13''' </font>
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LP
+
JL, LP
-
* Performed "Plating to Check for Plaque Size" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Purified the propagated mutant from [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
 +
 
 +
* Started approximately 20 mL of E coli B liquid culture overnight.
<br>
<br>
-
<font size="4"> '''9/23/13''' </font>
+
<font size="4"> '''10/10/13''' </font>
-
JL, LP
+
LP
-
* Performed "Plating to Check for Plaque Size 2" and "Checking for Plaque Viability from Step 5" for  [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Performed a spot to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
 +
 
 +
* Started approximately 20 mL of E coli B liquid culture overnight.
<br>
<br>
-
<font size="4"> '''9/24/13''' </font>
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<font size="4"> '''10/11/13''' </font>
-
LP
+
JL, LP
-
* Started approximately 30 mL E coli B liquid culture overnight.
+
* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
<br>
<br>
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<font size="4"> '''9/25/13''' </font>
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<font size="4"> '''10/12/13''' </font>
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JL, LP
+
JL
-
* Performed "Checking for Plaque Viability 2" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+
* Started approximately 15mL of E coli B liquid culture overnight
-
 
+
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* Started approximately 25mL of E coli B liquid culture overnight
+
<br>
<br>
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<font size="4"> '''9/26/13''' </font>
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<font size="4"> '''10/13/13''' </font>
-
LP
+
JL
-
* Started approximately 25mL of E. coli B overnight.
+
* Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
<br>
<br>

Revision as of 00:40, 14 October 2013


Small Phage September - October Notebook: October 1 - October 15 Daily Log



Small Phage
March-April
May-June
July-August
September-October

10/1/13 - 10/3/13

JL, LP

  • Worked on putting together Jamboree presentation and poster.


10/4/13 - 10/6/13

JL, LP

  • North America Regional Jamboree! For more click here.


10/7/13

JL

  • Started approximately 10 mL E coli B liquid culture overnight.


10/8/13

JL


10/9/13

JL, LP

  • Started approximately 20 mL of E coli B liquid culture overnight.


10/10/13

LP

  • Started approximately 20 mL of E coli B liquid culture overnight.


10/11/13

JL, LP


10/12/13

JL

  • Started approximately 15mL of E coli B liquid culture overnight


10/13/13

JL


9/27/13

JL, LP

  • Finished updating the wiki.
  • Started approximately 30mL of E. coli B overnight.


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