Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period3/Dailylog

From 2013.igem.org

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LP
LP
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* Performed a spot to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Performed spot tests to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
* Started approximately 20 mL of E coli B liquid culture overnight.
* Started approximately 20 mL of E coli B liquid culture overnight.
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JL
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* Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)
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* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)
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Revision as of 00:50, 14 October 2013


Small Phage September - October Notebook: October 1 - October 15 Daily Log



Small Phage
March-April
May-June
July-August
September-October

10/1/13 - 10/3/13

JL, LP

  • Worked on putting together Jamboree presentation and poster.
  • Tried to take EM to verify mutant size - did not work very well: phage titer is too low


10/4/13 - 10/6/13

JL, LP

  • North America Regional Jamboree! For more information click here.


10/7/13

JL

  • Started approximately 10 mL E coli B liquid culture overnight.


10/8/13

JL


10/9/13

JL, LP

  • Started approximately 20 mL of E coli B liquid culture overnight.


10/10/13

LP

  • Started approximately 20 mL of E coli B liquid culture overnight.

JL

  • Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)


10/11/13

JL, LP

  • Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)


10/12/13

JL

  • Started approximately 15mL of E coli B liquid culture overnight


10/13/13

JL


9/27/13

JL, LP

  • Finished updating the wiki.
  • Started approximately 30mL of E. coli B overnight.