Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog

From 2013.igem.org

(Difference between revisions)

Revision as of 03:24, 29 October 2013

Small Phage September - October Notebook: October 16 - October 31 Daily Log

Small Phage

10/16/13

JL, LP

Plated for mutants as part of 10.8 Characterization of Mutant Phage.

10/18/13

JL, LP

Performed amplification and purification of insert as part of 10.18 Cloning T7 Capsid Protein into iGEM Registry.

10/19/13

JL, LP

Ran a low melt gel for 10.18 Cloning T7 Capsid Protein into iGEM Registry.

10/20/13

JL

Performed the repeat PCR and gel step in 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/21/13

JL, LP

Did restriction digest for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/22/13

JL

Low-melt gel for purification of sticky-ended insert/vector for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/23/13

JL, LP

Did ligation and transformation for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/24/13

JL, LP

Did Colony PCR and Overnights for Vector Purification for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/25/13

JL, LP

Did plasmid cleanup for 10.18 Cloning T7 Capsid Protein into iGEM Registry and sent the plasmid into iGEM!

10/27/13

JL

Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage.

10/28/13

JL

Propagated WT, S4, S10, S21, S22, and L8 for 10/27/13

JL, LP

Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage.

Mapped out mutation for S4 capsid protein.

Finished the wiki.

@@ Line 66: / Line 66: @@
 <br>
-<font size="4"> '''10/9/13''' </font>
+<font size="4"> '''10/21/13''' </font>
 JL, LP
-* Purified the propagated mutant from [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Did restriction digest for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
-* Started approximately 20 mL of E coli B liquid culture overnight.
 <br>
-<font size="4"> '''10/10/13''' </font>
+<font size="4"> '''10/22/13''' </font>
-LP
+JL
-* Performed spot tests to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Low-melt gel for purification of sticky-ended insert/vector for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
-* Started approximately 20 mL of E coli B liquid culture overnight.
+<br>
-JL
+<font size="4"> '''10/23/13''' </font>
-* Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)
+JL, LP
+* Did ligation and transformation for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
 <br>
-<font size="4"> '''10/11/13''' </font>
+<font size="4"> '''10/24/13''' </font>
 JL, LP
-* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Did Colony PCR and Overnights for Vector Purification for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
-* Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)
 <br>
-<font size="4"> '''10/12/13''' </font>
+<font size="4"> '''10/25/13''' </font>
-JL
+JL, LP
-* Started approximately 15mL of E coli B liquid culture overnight
+* Did plasmid cleanup for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]] and sent the plasmid into iGEM!
 <br>
-<font size="4"> '''10/13/13''' </font>
+<font size="4"> '''10/27/13''' </font>
 JL
-* Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
+* Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
 <br>
-<font size="4"> '''9/27/13''' </font>
+<font size="4"> '''10/28/13''' </font>
+JL
+* Propagated WT, S4, S10, S21, S22, and L8 for <font size="4"> '''10/27/13''' </font>
 JL, LP
-* Determined results for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
+* Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
-* Finished updating the wiki.
+* Mapped out mutation for S4 capsid protein.
-* Started approximately 30mL of E. coli B overnight.
+* Finished the wiki.
+<br>
 <br>