Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size
From 2013.igem.org
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* To see a statistical significance in plaque sizes between different phages, many plaques are needed. Thus 10 plates were used for T1, T2, T3, and T7. Because T4 form much smaller plaques, only 3 plates were used. | * To see a statistical significance in plaque sizes between different phages, many plaques are needed. Thus 10 plates were used for T1, T2, T3, and T7. Because T4 form much smaller plaques, only 3 plates were used. | ||
- | * Experimental procedures are as follow: 0.5mL of E coli | + | * Experimental procedures are as follow: 0.5mL of E coli B (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 24 hours. |
2) Propagation and spot test of T1 (8.12-8.20) | 2) Propagation and spot test of T1 (8.12-8.20) |
Revision as of 20:01, 9 September 2013
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8.16 Modeling phage plaque size - Experiment One
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Plating (8.16)
2) Propagation and spot test of T1 (8.12-8.20)
3) Spot test for T2 from stock (8.20)
4) Preliminary Titer (9.8)
V) Results 1) Plating
2) T1 and T2 spot test
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