Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size
From 2013.igem.org
(Difference between revisions)
Line 64: | Line 64: | ||
* Dilution series -2 through -12 was performed for T1. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar. | * Dilution series -2 through -12 was performed for T1. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar. | ||
+ | |||
+ | 3) Spot test for T2 from stock (8.20) | ||
+ | |||
+ | * Dilution series -2 through -7 was performed for T2 from stock. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar. | ||
'''V) Results''' | '''V) Results''' | ||
Line 87: | Line 91: | ||
|- | |- | ||
|T1 | |T1 | ||
- | | | + | | |
- | | | + | |contamination |
- | | | + | | |
- | | | + | | |
| | | | ||
| | | | ||
Line 96: | Line 100: | ||
|- | |- | ||
|T2 | |T2 | ||
- | | | + | | |
- | | | + | | |
- | | | + | | |
- | | | + | | |
| | | | ||
| | | | ||
Line 105: | Line 109: | ||
|- | |- | ||
|T3 | |T3 | ||
- | | | + | | |
- | | | + | | |
- | | | + | | |
- | | | + | | |
| | | | ||
| | | | ||
Line 114: | Line 118: | ||
|- | |- | ||
|T4 | |T4 | ||
- | | | + | | |
- | | | + | | |
- | | | + | | |
- | | | + | | |
| | | | ||
| | | | ||
Line 123: | Line 127: | ||
|- | |- | ||
|T7 | |T7 | ||
- | | | + | | |
- | | | + | | |
- | | | + | | |
- | | | + | | |
| | | | ||
| | | | ||
Line 133: | Line 137: | ||
</center> | </center> | ||
+ | |||
+ | 2) T1 and T2 spot test | ||
+ | |||
+ | * T1 showed plaques up to -8 after propagation, while -2 showed plaques up to -4. | ||
Revision as of 21:21, 1 September 2013
| |||||||||||||||||||||||||||||||||||||||||||
|
8.16 Modeling phage plaque size - Experiment One
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Plating (8.16)
2) Propagation and spot test of T1 (8.12-8.20)
3) Spot test for T2 from stock (8.20)
V) Results 1) Plating
2) T1 and T2 spot test
|