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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period1/Dailylog
From 2013.igem.org
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- Made 750mL of x8 top agar. | - Made 750mL of x8 top agar. | ||
| - | - We started our fourth round of mutagenesis in | + | |
| + | - We started our fourth round of mutagenesis in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]] | ||
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Revision as of 19:54, 8 July 2013
| Small Phage July - August Notebook: July 1 - July 15 Daily Log
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7/1/13 - Started approximately 30mL of BL21 liquid culture overnight.
7/2/13 - Performed second round of titer for 6.27 Mutagen Concentration Test - Third Protocol, repeating the procedures from last Friday. - Started approximately 50mL of BL21 liquid culture overnight.
7/3/13 - Performed spot test for 6.27 Mutagen Concentration Test - Third Protocol to determine phage viability. 5.3 stock and phage from 6.12 Mutagen Concentration Test - Second Protocol were used as positive control. - Worked on the introduction for our outreach presentation.
7/7/13 - Started 60mL of BL21 liquid culture overnight.
7/8/13 - Made 750mL of x8 top agar. - We started our fourth round of mutagenesis in 7.8 Mutagen Concentration Test - Fourth Protocol
6/26/13 - Made about 50ml of BL21 liquid culture overnight
6/27/13 - Started the mutagenesis procedure for 6.27 Mutagen Concentration Test - Third Protocol
6/28/13 - Performed first round of titer for 6.27 Mutagen Concentration Test - Third Protocol. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.
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