Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period1/Dailylog
From 2013.igem.org
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- | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage July - August Notebook: July 1 - July 15 Daily Log'''</font> | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> |
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+ | : '''Small Phage July - August Notebook: July 1 - July 15 Daily Log'''</font> | ||
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+ | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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<font size="4"> '''7/1/13''' </font> | <font size="4"> '''7/1/13''' </font> | ||
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- Performed spot test for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]] to determine phage viability. 5.3 stock and phage from [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]] were used as positive control. | - Performed spot test for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]] to determine phage viability. 5.3 stock and phage from [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]] were used as positive control. | ||
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+ | - Worked on the introduction for our outreach presentation. | ||
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- | <font size="4"> ''' | + | <font size="4"> '''7/7/13''' </font> |
- | - Started | + | - Started 60mL of BL21 liquid culture overnight. |
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- | <font size="4"> ''' | + | <font size="4"> '''7/8/13''' </font> |
- | - Made | + | - Made 750mL of x8 top agar. |
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+ | - We started our fourth round of mutagenesis in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]] | ||
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- | <font size="4"> ''' | + | <font size="4"> '''7/9/13''' </font> |
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+ | - Outreach @ Orem Public Library! | ||
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+ | - Started approximately 20mL of E coli BL21 liquid culture overnight. | ||
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+ | <br> | ||
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+ | <font size="4"> '''7/10/13''' </font> | ||
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+ | - Performed titer to determine mutated phage concentration for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]] | ||
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+ | <br> | ||
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+ | <font size="4"> '''7/11/13''' </font> | ||
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+ | - Started approximately 10mL of E coli BL21 liquid culture overnight. | ||
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+ | <br> | ||
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+ | <font size="4"> '''7/12/13''' </font> | ||
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+ | - Performed spot test to confirm the titer result from July 10th. Detail procedure listed in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol|7.8 Mutagen Concentration Test - Fourth Protocol]]. | ||
- | - | + | - Started approximately 6 mL of E coli liquid culture overnight. |
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- | <font size="4"> ''' | + | <font size="4"> '''7/13/13''' </font> |
- | - | + | - Set up the phage liquid culture for propagation in [[Team:BYU Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13 Phage Propagation|7.13 Phage Propagation Experiment]]. |
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- | <font size="4"> ''' | + | <font size="4"> '''7/14/13''' </font> |
- | - | + | - Started approximately 30mL of E coli BL21 liquid culture overnight. |
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Latest revision as of 18:15, 26 August 2013
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7/1/13 - Started approximately 30mL of BL21 liquid culture overnight.
7/2/13 - Performed second round of titer for 6.27 Mutagen Concentration Test - Third Protocol, repeating the procedures from last Friday. - Started approximately 50mL of BL21 liquid culture overnight.
7/3/13 - Performed spot test for 6.27 Mutagen Concentration Test - Third Protocol to determine phage viability. 5.3 stock and phage from 6.12 Mutagen Concentration Test - Second Protocol were used as positive control. - Worked on the introduction for our outreach presentation.
7/7/13 - Started 60mL of BL21 liquid culture overnight.
7/8/13 - Made 750mL of x8 top agar. - We started our fourth round of mutagenesis in 7.8 Mutagen Concentration Test - Fourth Protocol
7/9/13 - Outreach @ Orem Public Library! - Started approximately 20mL of E coli BL21 liquid culture overnight.
7/10/13 - Performed titer to determine mutated phage concentration for 7.8 Mutagen Concentration Test - Fourth Protocol
7/11/13 - Started approximately 10mL of E coli BL21 liquid culture overnight.
7/12/13 - Performed spot test to confirm the titer result from July 10th. Detail procedure listed in 7.8 Mutagen Concentration Test - Fourth Protocol. - Started approximately 6 mL of E coli liquid culture overnight.
7/13/13 - Set up the phage liquid culture for propagation in 7.13 Phage Propagation Experiment.
7/14/13 - Started approximately 30mL of E coli BL21 liquid culture overnight.
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