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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period1/Dailylog
From 2013.igem.org
| Small Phage May - June Notebook: June 17 - June 30 Daily Log
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7/1/13 - Started approximately 30mL of BL21 liquid culture overnight.
7/2/13 - Performed second round of titer for 6.27 Mutagen Concentration Test - Third Protocol, repeating the procedures from last Friday. - Started approximately 50mL of BL21 liquid culture overnight.
7/3/13 - Performed spot test for 6.27 Mutagen Concentration Test - Third Protocol to determine phage viability. 5.3 stock and phage from 6.12 Mutagen Concentration Test - Second Protocol were used as positive control.
6/23/13 - Started 40mL of BL21 liquid culture overnight.
6/24/13 - Made more x8 top agar. - Because the large plaque confirmation 1 had contamination from overnight (the LB was contaminated the next day), we redid the confirmation procedure: Large Plaque Confirmation 2 in 6.12 Mutagen Concentration Test - Second Protocol.
6/26/13 - Made about 50ml of BL21 liquid culture overnight
6/27/13 - Started the mutagenesis procedure for 6.27 Mutagen Concentration Test - Third Protocol
6/28/13 - Performed first round of titer for 6.27 Mutagen Concentration Test - Third Protocol. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.
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