Team:BostonU/NotebookML

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Characterization Notebook

Goal: Develop a well-characterized library of parts for the MoClo Assembly Method

Subgoals:
  • Assemble level 0 parts needed
    • Constitutive Promoter Characterization Project:
      • J23100_AB, J23113_AB, J23115_AB, J23115_EB, J23116_AB, J23116_EB, J23117_AB, J23118_AB, J23119_AB
      • BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, BCD16_BC: completed 6/27/13
    • Repressible Promoter Characterization Project:
      • C0012 (LacI), C0062_CD (LuxR), C0071_CD (rhlR_CD), pLacI (R0010_FB)
  • Assemble level 1 circuits for characterization of Constitutive Promoter Library
    • 160 circuits that have different combinations of J231XX promoters and RBS’s
    • 1 set with all promoters + BCD2
    • 1 set with all RBS’s+J23100
  • Assemble level 1 circuits for characterization of Repressible Promoter
  • Run flow cytometry on the promoter characterization circuits
  • Assemble level 2 inverter as an example of the library’s use
  • Switch the backbone in some existing level 1 parts to study how different antibiotics affect the flow cytometry data: 6/21/13

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