Team:BostonU/PUP

From 2013.igem.org

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<b>June 5, 2013</b>
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Design to Direction
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compare contrast doing things via puppeteer vs. human (~100-200 parts in two weeks?)
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hope to reduce error and
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could use tecan to switch out backbone.
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maybe create basic moclo XFP transcription units
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Robot does:
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Moclo rxn
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Transformation
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Ligation
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Restriction Digest
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resource tracking, when to replace
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Come up with hypothesis of human vs. machine
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Efficiency of Robot,? Puppeteer speed up planning for robot? Error of human and reaction failure?
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For human error - have a partner checking for errors
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Human errors
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- setting plates up wrong( have partners)
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- make sure dilutions are right (excel errors? have partners do excel together)
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-just always have partners.
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-don’t rush yo
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Protocols must be adapted to go with puppeteer/robot. Testing to optimize.
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Aiming to test by making ten devices in duplicate
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Target for Tuesday June 11
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figure out steps of protocol for robot in excel sheet with exact buffer/enz/etc.
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10 devices
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ADD WHITEBOARD PICTURE FROM DROPBOX FOLDER
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<b>June 11, 2013</b>
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See picture for parts that need to be made * 4
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dilute to 25 fmol
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Janoo sent working csv files for Raven
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ADD WHITEBOARD PICTURE FROM DROPBOX FOLDER
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<b>June 18, 2013</b>
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download arcs  file from Raven.
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Arcs = raven to puppeteer
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upload arc file into puppeteer
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In puppeteer, select arc file, and personnel involved.
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→ then assembly graph and protocol graph generated
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*went over process in the assembly graph.
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next step: know details of the protocols themselves.
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require the specific instruction of the protocol. more detailed than excel sheets and how to allocate resources. how much and what resources are needed to go from 1 rxn to 10 rxn etc
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Want higher level details--> pick up this tube, etc
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labels are 8 letter labels. generated in raven/puppeteer that needs to be written on the container. (randomly generated), doesn’t tell us about the contents of the sample
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Showed current naming protocol for L1
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*brain storming about labeling*
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maybe send out a survey to wetlab about labeling
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Revision as of 16:48, 20 June 2013

BostonU iGEM Team: Welcome