Team:BostonU/PUP

From 2013.igem.org

(Difference between revisions)
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<b>June 5, 2013</b>
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<b>June 5, 2013</b><br>
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Design to Direction
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Design to Direction<br>
compare contrast doing things via puppeteer vs. human (~100-200 parts in two weeks?)
compare contrast doing things via puppeteer vs. human (~100-200 parts in two weeks?)
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hope to reduce error and  
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<br> hope to reduce error and  
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could use tecan to switch out backbone.
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<br> could use tecan to switch out backbone.
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maybe create basic moclo XFP transcription units
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<br>maybe create basic moclo XFP transcription units
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Robot does:
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<br>Robot does:
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Moclo rxn
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<br>Moclo rxn
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Transformation
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<br>Transformation
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Ligation
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<br>Ligation
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Restriction Digest
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<br>Restriction Digest
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resource tracking, when to replace
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<br>resource tracking, when to replace
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<br><br>
Come up with hypothesis of human vs. machine
Come up with hypothesis of human vs. machine
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Efficiency of Robot,? Puppeteer speed up planning for robot? Error of human and reaction failure?
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<br> Efficiency of Robot? Puppeteer speed up planning for robot? Error of human and reaction failure?
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For human error - have a partner checking for errors
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<br>For human error - have a partner checking for errors
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<br><br>
Human errors  
Human errors  
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- setting plates up wrong( have partners)
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<br>- setting plates up wrong(have partners)
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- make sure dilutions are right (excel errors? have partners do excel together)
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<br>- make sure dilutions are right (excel errors? have partners do excel together)
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-just always have partners.  
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<br>- just always have partners.  
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-don’t rush yo
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<br>- don’t rush
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<br><br>
Protocols must be adapted to go with puppeteer/robot. Testing to optimize.  
Protocols must be adapted to go with puppeteer/robot. Testing to optimize.  
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Aiming to test by making ten devices in duplicate
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<br>Aiming to test by making ten devices in duplicate
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<br><br>
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Target for Tuesday June 11
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<i>Target for Tuesday June 11</i>
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figure out steps of protocol for robot in excel sheet with exact buffer/enz/etc.  
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<br>figure out steps of protocol for robot in excel sheet with exact buffer/enz/etc.  
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10 devices
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<br>10 devices
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<br>
ADD WHITEBOARD PICTURE FROM DROPBOX FOLDER
ADD WHITEBOARD PICTURE FROM DROPBOX FOLDER
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<br><br><br>
<b>June 11, 2013</b>
<b>June 11, 2013</b>
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See picture for parts that need to be made * 4
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<br>See picture for parts that need to be made * 4
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dilute to 25 fmol
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<br>dilute to 25 fmol
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<br><br>
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Janoo sent working csv files for Raven
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<br>Janoo sent working csv files for Raven
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<br><br>
ADD WHITEBOARD PICTURE FROM DROPBOX FOLDER
ADD WHITEBOARD PICTURE FROM DROPBOX FOLDER
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<br><br><br>
<b>June 18, 2013</b>
<b>June 18, 2013</b>
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download arcs  file from Raven.  
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<br>download arcs  file from Raven.  
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Arcs = raven to puppeteer  
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<br>Arcs = raven to puppeteer  
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upload arc file into puppeteer
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<br>upload arc file into puppeteer
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In puppeteer, select arc file, and personnel involved.  
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<br>In puppeteer, select arc file, and personnel involved.  
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→ then assembly graph and protocol graph generated
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<br>→ then assembly graph and protocol graph generated
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*went over process in the assembly graph.  
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<br>*went over process in the assembly graph.  
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<br>
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next step: know details of the protocols themselves.
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<br><i>next step: know details of the protocols themselves</i>
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require the specific instruction of the protocol. more detailed than excel sheets and how to allocate resources. how much and what resources are needed to go from 1 rxn to 10 rxn etc
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<br>require the specific instruction of the protocol. more detailed than excel sheets and how to allocate resources. <br>how much and what resources are needed to go from 1 rxn to 10 rxn etc
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<br><br>
Want higher level details--> pick up this tube, etc
Want higher level details--> pick up this tube, etc
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<br><br>
labels are 8 letter labels. generated in raven/puppeteer that needs to be written on the container. (randomly generated), doesn’t tell us about the contents of the sample
labels are 8 letter labels. generated in raven/puppeteer that needs to be written on the container. (randomly generated), doesn’t tell us about the contents of the sample
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<br><br>
Showed current naming protocol for L1
Showed current naming protocol for L1
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*brain storming about labeling*
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<br>*brain storming about labeling*
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maybe send out a survey to wetlab about labeling  
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<br>maybe send out a survey to wetlab about labeling  
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Revision as of 16:53, 20 June 2013

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