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Team:Braunschweig/Notebook
From 2013.igem.org
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<div id="Week1" class="menuSection"> | <div id="Week1" class="menuSection"> | ||
<h2><a href="#Week1">Week 1: May 19 - May 26, 2013</a></h2> | <h2><a href="#Week1">Week 1: May 19 - May 26, 2013</a></h2> | ||
| - | <p><p style=" margin-left:5px; margin-right:5px;">We set up our labspace and started some preparatory work.</p> | + | <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:0px; padding:0px">We set up our labspace and started some preparatory work.</p> |
| - | <p><p style="font-size: | + | <p><p style="font-size:13px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; padding:0px; margin-right:5px; margin-top:0px; margin-bottom:0px">Tuesday, May 21, 2013</p> |
| + | <p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px; padding:0px;"> <img alt="May21" src="https://static.igem.org/mediawiki/2013/a/a3/Braunschweig_Lab_Journal_May_21.jpg" width="400" align="right" vspace="0" hspace="20"/> | ||
| + | We finally moved in our lab. A bit of dust here and some rubbish to dispose there, but after a few hours of combined strength our lab was ready to go. Wet experiments can begin!</p> | ||
| + | |||
| + | <p><p style="font-size:13px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px";padding:0px;>Thursday, May 23, 2013</p> | ||
| + | |||
| + | <p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px"> | ||
| + | <b>Investigators: Kevin, Kerstin, Laura</b><br> | ||
| + | We prepared some chemically competent XL1 blue E. Coli cells for all the transformations we are going to have to do during the project.</p> | ||
| + | |||
| + | <p><p style="font-size:13px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Friday, May 24, 2013</p> | ||
| + | |||
| + | <p><p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px"> | ||
| + | <b>Investigators: Kevin, Kerstin, Laura</b><br> | ||
| + | Today we did test transformations with our freshly prepared competent cells. We used the plasmids pUC 18 and pUC19 to calculate the transformation efficiency. 100 pg of DNA were used for each transformation with 50 µl cells. | ||
| + | Additionally, competent cells were plated on ampicillin, kanamycin and chloramphenicol to make sure that the strain does not carry any corresponding resistance.</p> | ||
</div> | </div> | ||
Revision as of 22:14, 9 September 2013
Labjournal
This is the documentation of our lab work. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.
Week 1: May 19 - May 26, 2013
We set up our labspace and started some preparatory work.
Tuesday, May 21, 2013
We finally moved in our lab. A bit of dust here and some rubbish to dispose there, but after a few hours of combined strength our lab was ready to go. Wet experiments can begin!
Thursday, May 23, 2013
Investigators: Kevin, Kerstin, Laura
We prepared some chemically competent XL1 blue E. Coli cells for all the transformations we are going to have to do during the project.
Friday, May 24, 2013
Investigators: Kevin, Kerstin, Laura
Today we did test transformations with our freshly prepared competent cells. We used the plasmids pUC 18 and pUC19 to calculate the transformation efficiency. 100 pg of DNA were used for each transformation with 50 µl cells.
Additionally, competent cells were plated on ampicillin, kanamycin and chloramphenicol to make sure that the strain does not carry any corresponding resistance.
