Team:Braunschweig/Notebook

From 2013.igem.org

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<p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Tuesday, June 25, 2013</p>
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<p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Wednesday, June 25, 2013</p>
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<b>Investigators: Jan, Kerstin </b><br>
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<b>Investigator: Jan, Kerstin</b><br>
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<p><img alt="Soil samples" src="https://static.igem.org/mediawiki/2013/e/ea/Braunschweig_Lab_Week6_Bodenproben.jpg" height="200px" align="left", vspace="0", hspace="20", margin-right:20px/></p>
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<img alt="Soil samples" src="https://static.igem.org/mediawiki/2013/e/ea/Braunschweig_Lab_Week6_Bodenproben.jpg" height="200px" align="left", vspace="0", hspace="20", margin-right:20px/></p>
We made minipreps and glycerol stocks of R0071/R0062/K091117-B0032-<i>ampR</i>. For validation of K091117’s sequence (2 basepairs differed from the sequence given in the registry) Kerstin conducted another Phusion-PCR. After ligation the product was evaluated by colony PCR. Together with R0071/R0062/K091117-B0032-<i>ampR</i>, K091117 was prepared and sent for sequencing. K091117-B0032-<i>ampR</i> was a double clone.<br>  
We made minipreps and glycerol stocks of R0071/R0062/K091117-B0032-<i>ampR</i>. For validation of K091117’s sequence (2 basepairs differed from the sequence given in the registry) Kerstin conducted another Phusion-PCR. After ligation the product was evaluated by colony PCR. Together with R0071/R0062/K091117-B0032-<i>ampR</i>, K091117 was prepared and sent for sequencing. K091117-B0032-<i>ampR</i> was a double clone.<br>  
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We also took soil samples for the iGEM Team Norwich.</p>
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We also took soil samples for the iGEM Team Norwich.
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Revision as of 22:38, 4 October 2013

Labjournal

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Braunschweig This is the documentation of our lab work. Achievements of each week are summerized followed by a daily discription of our experiments.

An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our
Attributions section for efforts beyond the lab work.






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