Team:Buenos Aires/ summary

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<center style="font-family:'Vollkorn', serif; color: white;"> Wetlab work at UBA labs </center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.</center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.</center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.</center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">Al being lit by bioluminescent bacteria! </center></div>
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Revision as of 21:27, 27 September 2013

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012


Wetlab work at UBA labs
We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.
Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.
We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.
Al being lit by bioluminescent bacteria!


To Drink Or Not To Drink

Contamination is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.

At present, the spatial and temporal quantification of contaminants is limited by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain information. Our central goal is to help solve this situation, so we thought of a biosensor that is cheap and easy to use by people without previous training or equipment.


To Drink or Not To Drink
File:Howworks.gif BairesGrupal.jpg

Arsenic is naturally present in soil and ground water in certain regions. Argentina, the United States, Bangladesh and Chile are on the top of the list of countries with high natural arsenic concentration in ground water of certain zones. Population without access to treated water living in these areas is exposed to Arsenic consumption. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders.

Mapa mundial arsenico.jpg