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Team:Chiba/Notebook
From 2013.igem.org
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<b>8/22//2013</b><br> | <b>8/22//2013</b><br> | ||
| - | + | .Extracting the human ferritin plasmid and iron chaperone plasmid | |
<b>8/23/2013</b><br> | <b>8/23/2013</b><br> | ||
| - | + | .BBa_I746908 and BBa_I746902 transformation | |
<b>8/24/2013</b><br> | <b>8/24/2013</b><br> | ||
| - | + | .Extracting BBa_I746908 and BBa_I746902 | |
<b>8/25/2013</b><br> | <b>8/25/2013</b><br> | ||
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<b>8/29/2013</b><br> | <b>8/29/2013</b><br> | ||
| + | .Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)<br> | ||
| + | .PCR:(4-6c),ferritin(mid,strong)<br> | ||
| + | .Digestion test:4-6c,ferritin,iron chaperone<br> | ||
| + | .Pre-culture:CRISPRi<br> | ||
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<b>8/30/2013</b><br> | <b>8/30/2013</b><br> | ||
Revision as of 07:15, 10 September 2013
week1(8/18/2013~8/25/2013)
8/22//2013
.Extracting the human ferritin plasmid and iron chaperone plasmid
8/23/2013
.BBa_I746908 and BBa_I746902 transformation
8/24/2013
.Extracting BBa_I746908 and BBa_I746902
8/25/2013
week2(8/26/2013~9/1/2013)
8/26/2013
8/27/2013
8/28/2013
8/29/2013
.Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)
.PCR:(4-6c),ferritin(mid,strong)
.Digestion test:4-6c,ferritin,iron chaperone
.Pre-culture:CRISPRi
8/30/2013
8/31/2013
9/1/2013
.Begining editing Wiki
week3(9/2/2013~9/7/2013)
9/2/2013
.Making plates with Broth and methylene blue(MB)
9/3/2013
9/4/2013
9/5/2013
9/6/2013
9/7/2013
