Team:Chiba/Notebook
From 2013.igem.org
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<p> | <p> | ||
<b>8/22//2013</b><br> | <b>8/22//2013</b><br> | ||
- | .Extracting the human ferritin plasmid and iron chaperone plasmid | + | .Extracting the human ferritin plasmid and iron chaperone plasmid<br> |
<b>8/23/2013</b><br> | <b>8/23/2013</b><br> | ||
- | .BBa_I746908 and BBa_I746902 transformation | + | .BBa_I746908 and BBa_I746902 transformation<br> |
<b>8/24/2013</b><br> | <b>8/24/2013</b><br> | ||
- | .Extracting BBa_I746908 and BBa_I746902 | + | .Extracting BBa_I746908 and BBa_I746902<br> |
<b>8/25/2013</b><br> | <b>8/25/2013</b><br> | ||
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<b>8/30/2013</b><br> | <b>8/30/2013</b><br> | ||
- | + | .Extracting CRISPRi plasmids(fur,fieF,gor,and trxB)<br> | |
+ | .PCR:ferritin(min,strong),CRISPRi(fur,fieF,for,and trxB)<br> | ||
+ | .Gel electrophresis:PCR products<br> | ||
+ | .Gel extractions:PCR products<br> | ||
<b>8/31/2013</b><br> | <b>8/31/2013</b><br> | ||
+ | .Digestion:ferritin(mid and strong),4-6c<br> | ||
<b>9/1/2013</b><br> | <b>9/1/2013</b><br> | ||
+ | .FASTR:CRISPRi<br> | ||
+ | .Ligation:ferritin and 4-6c<br> | ||
.Begining editing Wiki<br> | .Begining editing Wiki<br> | ||
Revision as of 07:38, 10 September 2013
week1(8/18/2013~8/25/2013)
8/22//2013
.Extracting the human ferritin plasmid and iron chaperone plasmid
8/23/2013
.BBa_I746908 and BBa_I746902 transformation
8/24/2013
.Extracting BBa_I746908 and BBa_I746902
8/25/2013
week2(8/26/2013~9/1/2013)
8/26/2013
8/27/2013
8/28/2013
8/29/2013
.Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)
.PCR:(4-6c),ferritin(mid,strong)
.Digestion test:4-6c,ferritin,iron chaperone
.Pre-culture:CRISPRi
8/30/2013
.Extracting CRISPRi plasmids(fur,fieF,gor,and trxB)
.PCR:ferritin(min,strong),CRISPRi(fur,fieF,for,and trxB)
.Gel electrophresis:PCR products
.Gel extractions:PCR products
8/31/2013
.Digestion:ferritin(mid and strong),4-6c
9/1/2013
.FASTR:CRISPRi
.Ligation:ferritin and 4-6c
.Begining editing Wiki
week3(9/2/2013~9/7/2013)
9/2/2013
.Making plates with Broth and methylene blue(MB)
9/3/2013
9/4/2013
9/5/2013
9/6/2013
9/7/2013