Team:Chiba/Notebook

From 2013.igem.org

(Difference between revisions)

Latest revision as of 04:22, 28 September 2013

iGEM-2013 Chiba

Assay

Reprogramming the iron homeostasis	Establishment of Iron storage system	Reprogramming the redox state of the cell

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 <body>
 <div class="textSpace">
-<div class="aside">
-<ul>
-<li><a href="#">Dairy</a></li>
-<li><a href="#">Method</a></li>
-</ul>
-</div>
@@ Line 86: / Line 79: @@
 <div class="article">
-<h3 id="c1"><a href="#c1">week1(8/18/2013~8/25/2013)</a></h3>
-<div>
-<p>
-<b>8/22//2013</b><br>
-.Extracting the human ferritin plasmid and iron chaperone plasmid<br>
-<b>8/23/2013</b><br>
-.BBa_I746908 and BBa_I746902 transformation<br>
-<b>8/24/2013</b><br>
-.Extracting BBa_I746908 and BBa_I746902<br>
-<b>8/25/2013</b><br>
-</p>
+<h2 style="background-color:#ff9933"><center>Assay</center></h2>
-</div>
-</div>
+<center>
+<table border="1">
-<div class="article">
-<h3 id="c2"><a href="#c2">week2(8/26/2013~9/1/2013)</a></h3>
-<div>
-<p>
-<b>8/26/2013</b><br>
-<b>8/27/2013</b><br>
+<tr>
-<b>8/28/2013</b><br>
+<td align="center">
+<a href="https://2013.igem.org/Team:Chiba/Assay/uptake"><img src = "https://static.igem.org/mediawiki/2013/archive/c/c2/20130927162646%21Chiba_uptake.jpg" ALT = "#"width="216.5px"height="349px"></A>
+</td>
-<b>8/29/2013</b><br>
-.Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)<br>
-.PCR:(4-6c),ferritin(mid,strong)<br>
-.Digestion test:4-6c,ferritin,iron chaperone<br>
-.Pre-culture:CRISPRi<br>
+<td align="center">
+<a href="https://2013.igem.org/Team:Chiba/Assay/store"><img src = "https://static.igem.org/mediawiki/2013/a/a3/Chiba_storage.jpg" ALT = "#"width="211px"height="346.5px"></a>
+</td>
-<b>8/30/2013</b><br>
+<td align="center">
-.Extracting CRISPRi plasmids(fur,fieF,gor,and trxB)<br>
+<a href="https://2013.igem.org/Team:Chiba/Assay/oxidation"><img src = "https://static.igem.org/mediawiki/2013/archive/8/8b/20130927163941%21Chiba_oxidation.jpg" ALT = "#"width="218px"height="349.5px"></a>
-.PCR:ferritin(min,strong),CRISPRi(fur,fieF,for,and trxB)<br>
+</td>
-.Gel electrophresis:PCR products<br>
-.Gel extractions:PCR products<br>
-<b>8/31/2013</b><br>
-.Digestion:ferritin(mid and strong),4-6c<br>
-<b>9/1/2013</b><br>
-.FASTR:CRISPRi<br>
-.Ligation:ferritin and 4-6c<br>
-.Begining editing　Wiki<br>
-</p>
-</div>
-</div>
+</tr>
-<div class="article">
+<tr>
-<h3 id="c3"><a href="#c3">week3(9/2/2013~9/7/2013)</a></h3>
-<div>
-<p>
-<b>9/2/2013</b><br>
-.Making plates with Broth and methylene blue(MB)<br>
-<b>9/3/2013</b><br>
-.Making olates with Broth and MB<br>
-.Culturing BL21 and shuffle , respectively<br>
-<b>9/4/2013</b><br>
-<b>9/5/2013</b><br>
+<th>
+<a href="https://2013.igem.org/Team:Chiba/Assay/uptake">Reprogramming the iron homeostasis</a>
+</th>
-<b>9/6/2013</b><br>
+<th>
+<a href="https://2013.igem.org/Team:Chiba/Assay/store">Establishment of Iron storage system</a>
+</th>
-<b>9/7/2013</b><br>
+<th>
+<a href="https://2013.igem.org/Team:Chiba/Assay/oxidation">Reprogramming the redox state of the cell</a>
+</th>
-</p>
+</tr>
-</div>
-</div>
-<div class="article">
-<h3 id="c4"><a href="#c4">week4(9/8/2013~9/14/2013)</a></h3>
-<div>
-<p>
-<b>9/9/2013</b><br>
-.Hold a presentation(state of progress)<br>
-.<br>
-<b>9/10/2013</b><br>
-<b>9/11/2013</b><br>
+</table>
+</center>
-<b>9/12/2013</b><br>
-<b>9/13/2013</b><br>
-<b>9/14/2013</b><br>
-</p>
-</div>
-</div>
-<div class="article">
-<h3 id="c5"><a href="#c5">week5(9/15/2013~9/21/2013)</a></h3>
-<div>
-<p>test</p>
-</div>
-</div>
-</div>