Team:Chiba/Notebook

From 2013.igem.org

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<li>test</li>
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<li><a href="#">Dairy</a></li>
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<li><a href="#">Method</a></li>
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<h3 id="c4"><a href="#c4">week4(9/8/2013~9/14/2013)</a></h3>
<h3 id="c4"><a href="#c4">week4(9/8/2013~9/14/2013)</a></h3>
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<p>test</p>
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<b>9/9/2013</b><br>
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.Hold a presentation(state of progress)<br>
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.
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<b>9/10/2013</b><br>
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<b>9/11/2013</b><br>
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<b>9/12/2013</b><br>
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<b>9/13/2013</b><br>
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<b>9/14/2013</b><br>
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Revision as of 07:53, 11 September 2013

iGEM-2013 Chiba

iGEM-2013 Chiba

week1(8/18/2013~8/25/2013)

8/22//2013
.Extracting the human ferritin plasmid and iron chaperone plasmid
8/23/2013
.BBa_I746908 and BBa_I746902 transformation
8/24/2013
.Extracting BBa_I746908 and BBa_I746902
8/25/2013

week2(8/26/2013~9/1/2013)

8/26/2013
8/27/2013
8/28/2013
8/29/2013
.Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)
.PCR:(4-6c),ferritin(mid,strong)
.Digestion test:4-6c,ferritin,iron chaperone
.Pre-culture:CRISPRi
8/30/2013
.Extracting CRISPRi plasmids(fur,fieF,gor,and trxB)
.PCR:ferritin(min,strong),CRISPRi(fur,fieF,for,and trxB)
.Gel electrophresis:PCR products
.Gel extractions:PCR products
8/31/2013
.Digestion:ferritin(mid and strong),4-6c
9/1/2013
.FASTR:CRISPRi
.Ligation:ferritin and 4-6c
.Begining editing Wiki

week3(9/2/2013~9/7/2013)

9/2/2013
.Making plates with Broth and methylene blue(MB)
9/3/2013
9/4/2013
9/5/2013
9/6/2013
9/7/2013

week4(9/8/2013~9/14/2013)

9/9/2013
.Hold a presentation(state of progress)
. 9/10/2013
9/11/2013
9/12/2013
9/13/2013
9/14/2013

week5(9/15/2013~9/21/2013)

test