Team:Chiba/Notebook

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iGEM-2013 Chiba

week1(8/18/2013~8/25/2013)

8/22//2013
.Extracting the human ferritin plasmid and iron chaperone plasmid 8/23/2013
.BBa_I746908 and BBa_I746902 transformation 8/24/2013
.Extracting BBa_I746908 and BBa_I746902 8/25/2013

week2(8/26/2013~9/1/2013)

8/26/2013
8/27/2013
8/28/2013
8/29/2013
.Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)
.PCR:(4-6c),ferritin(mid,strong)
.Digestion test:4-6c,ferritin,iron chaperone
.Pre-culture:CRISPRi
8/30/2013
8/31/2013
9/1/2013
.Begining editing　Wiki

week3(9/2/2013~9/7/2013)

9/2/2013
.Making plates with Broth and methylene blue(MB)
9/3/2013
9/4/2013
9/5/2013
9/6/2013
9/7/2013

week4(9/8/2013~9/14/2013)

test

week5(9/15/2013~9/21/2013)

test