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Team:Chiba/Project/oxidation
From 2013.igem.org
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| + | <h2 id="oxidation" style="background-color:#ff9933"><center>oxidation</center></h2> | ||
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| + | <p> | ||
| + | <i>E.coli</i> (wild type) can’t form the disulfide bonds because it has reductive state in cytosol. | ||
| + | <br> We use the shuffle cell whose genes, <i>trxB</i> and <i>gor</i> are knocked out. <i>TrxB</i> and <i>gor</i> codes reductase which … So shuffle cells have oxidative state enough to form the disulfide bonds. | ||
| + | </p> | ||
Revision as of 13:02, 20 September 2013
oxidation
E.coli (wild type) can’t form the disulfide bonds because it has reductive state in cytosol.
We use the shuffle cell whose genes, trxB and gor are knocked out. TrxB and gor codes reductase which … So shuffle cells have oxidative state enough to form the disulfide bonds.
