Team:Chiba/Project/oxidation

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iGEM-2013 Chiba

oxidation

E.coli (wild type) can’t form the disulfide bonds because it has reductive state in cytosol.
We use the shuffle cell whose genes, trxB and gor are knocked out. TrxB and gor codes reductase which … So shuffle cells have oxidative state enough to form the disulfide bonds.