Team:Chiba/Project/store

From 2013.igem.org

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<br>&nbsp;&nbsp;&nbsp;&nbsp;Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O<sub>2</sub>→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe<sub>2</sub>O<sub>3</sub>(H<sub>2</sub>O) has paramagnetism.
<br>&nbsp;&nbsp;&nbsp;&nbsp;Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O<sub>2</sub>→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe<sub>2</sub>O<sub>3</sub>(H<sub>2</sub>O) has paramagnetism.
<br>&nbsp;&nbsp;&nbsp;&nbsp;These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.</p>
<br>&nbsp;&nbsp;&nbsp;&nbsp;These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.</p>
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</br></br></br></br></br></br></br></br></br></br></br></br></p>
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</br></br></br></br></br></br></br></br></br></p>
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<h3 style="background-color:#ffdead ">2.1.Plasmid</h3>
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<h3 style="background-color:#ffdead ">2.Materials!&!Methods</h3>
<p>
<p>
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パーツの事書く<br><br><br><br><br>
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<h3 style="background-color:#f0ffff ">2.1.Parts</h3>
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<a href="#"> Parts link</a>
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<a href="#"> Part link</a>
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</p>
</p>
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<h3 style="background-color:#ffdead ">2.2.Expression check</h3>
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<h3 style="background-color:#f0ffff ">2.2.Expression check</h3>
<p>
<p>
SDS pageについて書く
SDS pageについて書く
</p>
</p>
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<h3 style="background-color:#f0ffff ">2.3.鉄耐性の評価</h3>
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<a href="#">Assay</a>
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<h3 style="background-color:#f0ffff ">2.4.磁性評価</h3>
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<a href="#">Assay</a>
<h3 style="background-color:#ffdead ">3.Results & Discussion</h3>
<h3 style="background-color:#ffdead ">3.Results & Discussion</h3>
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<h3 style="background-color:#f0ffff ">3.1.プラスミド</a>
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<p>
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<h3 style="background-color:#f0ffff ">3.2鉄耐性の評価</a>
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鉄を貯めるということは・・・・・
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</p>
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<br>
 
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<h3 style="background-color:#f0ffff ">3.3磁性評価</a>
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<h3 style="background-color:#f0ffff">3.1.鉄耐性評価</h3>
 
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<p>
 
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<a href="#">Assay</a>実験方法別ページ<br>
 
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実験結果書いてく
 
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</p>
 
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<h3 style="background-color:#f0ffff ">3.2.鉄取り込み量評価</h3>
 
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<p>
 
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<a href="#">Assay</a>実験方法別ページ<br>
 
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実験結果書いてく
 
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</p>
 
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<h3 style="background-color:#f0ffff">3.3.磁性評価</h3>
 
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<p>
 
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<a href="#">Assay</a>実験方法別ページ<br>
 
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</p>
 

Revision as of 18:11, 26 September 2013

iGEM-2013 Chiba

iGEM-2013 Chiba

Storage

1.Introduction

    Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
    Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.
    These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.










2.Materials!&!Methods

2.1.Parts

Parts link

2.2.Expression check

SDS pageについて書く

2.3.鉄耐性の評価

Assay

2.4.磁性評価

Assay

3.Results & Discussion

3.1.プラスミド

3.2鉄耐性の評価

3.3磁性評価

4.Conclusion

それは・・

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