Team:Chiba/Project/store

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Revision as of 01:27, 27 September 2013

iGEM-2013 Chiba

Storage

1.Introduction

    Fe(II) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
    Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(II)＋O₂→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(III) in ferritin. Fe₂O₃(H₂O) has paramagnetism.
    These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.

2.Materials!&!Methods

2.1.Parts

Parts link

2.2.Expression check

SDS pageについて書く

2.3.鉄耐性の評価

Assay

2.4.磁性評価

Assay

3.Results & Discussion

3.1.プラスミド

3.2鉄耐性の評価

3.3磁性評価

4.Conclusion

それは・・

@@ Line 21: / Line 21: @@
 <h3 style="background-color:#ffdead ">1.Introduction</h3>
-	<p>&nbsp;&nbsp;&nbsp;&nbsp;Fe(Ⅱ) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>.
+	<p>&nbsp;&nbsp;&nbsp;&nbsp;Fe(II) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>.
-	<br>&nbsp;&nbsp;&nbsp;&nbsp;Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O<sub>2</sub>→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe<sub>2</sub>O<sub>3</sub>(H<sub>2</sub>O) has paramagnetism.
+	<br>&nbsp;&nbsp;&nbsp;&nbsp;Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(II)＋O<sub>2</sub>→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(III) in ferritin. Fe<sub>2</sub>O<sub>3</sub>(H<sub>2</sub>O) has paramagnetism.
-	<br>&nbsp;&nbsp;&nbsp;&nbsp;These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.</p>
+	<br>&nbsp;&nbsp;&nbsp;&nbsp;These two effects enable isolation of iron in ferritin and produce <i>E.coli</i> with strong iron tolerance.</p>
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