From 2013.igem.org

17 September 2013

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Lab 223

Main purpose

The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.

Who was in the lab

Helen, Kasia, Ariadni

Procedure

Following the protocol Bioreactor.

Results

Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.

Time	Replicate 1	Replicate 2	Replicate 3	Control: Distilled Water
14:00	1.84 mg/L	1.64 mg/L	1.60 mg/L	N/A
22:00	1.43 mg/L	1.44 mg/L	1.50 mg/L	1.65 mg/L

At the end of the experiment the OD measurements were:

OD(end)	sample A	sample B	sample C
-	0.0255	0.1590	0.0801

Colorimetric results

Ranges

Measuring range 5-150 mg/L NH₄-N
Measuring range 0.02-1 mg/L NO₂-N

salt (mg/L)	sample A/start	sample A/end	sample B/start	sample B/end	sample C/start	sample C/end
ammonium	18	18	16	6	18	14
nitrite	0.93	0.91	0.92	1.72	0.9	0.9

Conclusion

There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.